SS-31 protocols?

[Blake41]

I tried MOTSc for about two weeks but didn't have any increased energy or anything. Then I realized I should have started with SS-31 first to prime my system so to speak so I stopped it LOL..... That's around the same time of my Tirz stall aka its never really worked for me.

So I came up with the plan to do a cycle of NAD & AOD between cycling off the Tirz. I do notice increased energy with the NAD+ as well, I was impressed since I've never had consistent responses from Sema or TIrz, FINALLY something my body is working with.

I'll start Reta January first, as long as I make it that long. Then I'll drop the AOD mid January and start the SS31, followed by the MOTSc....... I'll need a calendar to keep these things straight lol

Curious what are the doses per week when stacking NAD, MOTS-C and tirz? What did you find is working for you?

 

[Vicki]

I'm experimenting with 10mg ss31 alternating with 10mg motsc. In a study with ss31 the doses are very high. They gave 40mg to kids over 40kg body weight for condition described here.

Use of Elamipretide in patients assigned treatment in the compassionate use program: Case series in pediatric patients with rare orphan diseases - PMC

Several mitochondrial diseases are caused by pathogenic variants that impair membrane phospholipid remodeling, with no FDA‐approved therapies. Elamipretide targets the inner mitochondrial membrane where it binds to cardiolipin, resulting in improved ...

www.ncbi.nlm.nih.gov

 

[Blake41]

I'm experimenting with 10mg ss31 alternating with 10mg motsc. In a study with ss31 the doses are very high. They gave 40mg to kids over 40kg body weight for condition described here.

Use of Elamipretide in patients assigned treatment in the compassionate use program: Case series in pediatric patients with rare orphan diseases - PMC

Several mitochondrial diseases are caused by pathogenic variants that impair membrane phospholipid remodeling, with no FDA‐approved therapies. Elamipretide targets the inner mitochondrial membrane where it binds to cardiolipin, resulting in improved ...

www.ncbi.nlm.nih.gov

Do you think ss31 is needed with/before mots-c?

 

[Vicki]

Do you think ss31 is needed with/before mots-c?

I have no idea. I did ss31 4 weeks 10mg and then did motsc with Dr seeds protocol and saw no effect. I'm trying to alternate them now and see if there's any difference. Maybe my mitochondria is just fine though.

 

[RubiJeep_Girl]

Curious what are the doses per week when stacking NAD, MOTS-C and tirz? What did you find is working for you?

I only did two weeks of MOTSc and didn’t really feel any affects, I did 5mg every few days. Then I read you should start with SS31 first, so I stopped it.

Now with NAD+ twice weekly, I felt it during my workouts. I felt I could keep going, I could go longer. It’s a little boost of energy without feeling jittery. This week I’m increasing to three times a week.

I’ll pick up the SS31 & MOTSc in February after I finish this cycle of AOD and NAD+.

 

[MingHenry]

I've just started my 3d week of SS31 today. I stopped using lipo-c with NAD+ (Pegasus from Kimera Chems) for a few weeks before starting ss31 because I wanted to feel whatever there might be to feel with ss31, without external energy. And I'm very glad that I did. I plan on doing the three stages of this protocol - ss31/mots-c/Nad+ - in that order - and either for 8 weeks each or 12 weeks each. Though I have no scientific data to point to, I think that using ss31 not only cleans the mitochondria (whether you "feel" anything or not, it's working behind the scenes), but I think it also primes the cells for all the other peps you might research afterwards. I am doing ss31 daily, started low at 1mg, and have moved up to 3mg. I like how I feel - good clean energy and though I'm usually very focused, I do feel like my focus has become even more intense! Though the typical ss31 protocol seems to be 4mg off the bat, daily, running for 21 or 28 days or 1 month or 2 months or more, I wanted to move up slowly and I'm glad I am doing that too - so I started at 1mg, moved to 1.5mg, 2mg, 2.5mg, and now at 3mg over fourteen days. I think I'll stay at 3mg for the whole of this week and then reassess, staying at 3mg or moving up to 3.5mg. Once I decide whether to run ss31 for 8 or 12 weeks, I will add in mots-c about 3 weeks before I finish ss-31. The typical protocol for mots seems to be 5mg 3x a week, but I intend to start low there too, at 1mg again, to see how I react. I'll run mots-c, and increase dosing as feels right to me, drop the ss-31, continue the mots- and either at 8 weeks or 12, I'll introduce the NAD+, and again, I'll start low, not at 25mg with a fast titration schedule up, but at 10mg 3x a week, and move up slowly as I see fit. I can see keeping ss31 as a regular part of my pep protocols.

 

[scarywood75]

I've just started my 3d week of SS31 today. I stopped using lipo-c with NAD+ (Pegasus from Kimera Chems) for a few weeks before starting ss31 because I wanted to feel whatever there might be to feel with ss31, without external energy. And I'm very glad that I did. I plan on doing the three stages of this protocol - ss31/mots-c/Nad+ - in that order - and either for 8 weeks each or 12 weeks each. Though I have no scientific data to point to, I think that using ss31 not only cleans the mitochondria (whether you "feel" anything or not, it's working behind the scenes), but I think it also primes the cells for all the other peps you might research afterwards. I am doing ss31 daily, started low at 1mg, and have moved up to 3mg. I like how I feel - good clean energy and though I'm usually very focused, I do feel like my focus has become even more intense! Though the typical ss31 protocol seems to be 4mg off the bat, daily, running for 21 or 28 days or 1 month or 2 months or more, I wanted to move up slowly and I'm glad I am doing that too - so I started at 1mg, moved to 1.5mg, 2mg, 2.5mg, and now at 3mg over fourteen days. I think I'll stay at 3mg for the whole of this week and then reassess, staying at 3mg or moving up to 3.5mg. Once I decide whether to run ss31 for 8 or 12 weeks, I will add in mots-c about 3 weeks before I finish ss-31. The typical protocol for mots seems to be 5mg 3x a week, but I intend to start low there too, at 1mg again, to see how I react. I'll run mots-c, and increase dosing as feels right to me, drop the ss-31, continue the mots- and either at 8 weeks or 12, I'll introduce the NAD+, and again, I'll start low, not at 25mg with a fast titration schedule up, but at 10mg 3x a week, and move up slowly as I see fit. I can see keeping ss31 as a regular part of my pep protocols.

More info on ss31 and what you may have experienced (generated with scite.ai):

---

Based on the references provided, the key points about the use of SS31 peptide are:

- SS31 is a mitochondria-targeted antioxidant peptide that can inhibit apoptosis and oxidative stress by maintaining mitochondrial structure and function (1, 2, 3, 4). It has been shown to protect against various conditions involving mitochondrial dysfunction, such as sepsis-induced muscle weakness (2), atrial fibrillation (5), high glucose-induced neuronal damage (3), and tert-butylhydroperoxide-induced oxidative damage in retinal cells (4).

- SS31 has also been used in combination with other drugs or nanoparticles to enhance their therapeutic effects, such as in the treatment of myocardial ischemia-reperfusion injury (1, 6), Alzheimer's disease (7), and abdominal aortic aneurysm (8).

- The protective effects of SS31 have been attributed to its ability to reduce mitochondrial ROS production, stabilize mitochondrial membrane potential, and modulate signaling pathways like the p38 MAPK pathway (9, 10).

In summary, the references indicate that SS31 is a promising mitochondria-targeted antioxidant with a wide range of therapeutic applications, particularly in conditions involving mitochondrial dysfunction and oxidative stress.

References:

1. Zhang, Z., Chen, Z., Yang, L., Zhang, J., Li, Y., Li, C., … & Xiao, W. (2022). Platelet membrane–encapsulated msns loaded with ss31 peptide alleviate myocardial ischemia-reperfusion injury. *Journal of Functional Biomaterials, 13*(4), 181. https://doi.org/10.3390/jfb13040181

2. Supinski, G., Wang, L., Schroder, E., & Callahan, L. (2020). Ss31, a mitochondrially targeted antioxidant, prevents sepsis-induced reductions in diaphragm strength and endurance. *Journal of Applied Physiology, 128*(3), 463-472. https://doi.org/10.1152/japplphysiol.00240.2019

3. Cao, M., Jiang, J., Du, Y., & Yan, P. (2012). Mitochondria-targeted antioxidant attenuates high glucose-induced p38 mapk pathway activation in human neuroblastoma cells. *Molecular Medicine Reports, 5*(4), 929-934. https://doi.org/10.3892/mmr.2012.746

4. Ma, W., Zhu, X., Ding, X., Li, T., Hu, Y., Hu, X., … & Tang, S. (2015). Protective effects of ss31 on t-bhp induced oxidative damage in 661w cells. *Molecular Medicine Reports, 12*(4), 5026-5034. https://doi.org/10.3892/mmr.2015.4055

5. Wiersma, M., Marion, D., Wüst, R., Houtkooper, R., Zhang, D., Groot, N., … & Brundel, B. (2019). Mitochondrial dysfunction underlies cardiomyocyte remodeling in experimental and clinical atrial fibrillation. *Cells, 8*(10), 1202. https://doi.org/10.3390/cells8101202

6. Li, F., Liu, D., Liu, M., Q, J., Zhang, B., Mei, Q., … & Zhou, S. (2022). Tregs biomimetic nanoparticle to reprogram inflammatory and redox microenvironment in infarct tissue to treat myocardial ischemia reperfusion injury in mice. *Journal of Nanobiotechnology, 20*(1). https://doi.org/10.1186/s12951-022-01445-2

7. Qian, K., Bao, X., Li, Y., Wang, P., Guo, Q., Yang, P., … & Zhang, Q. (2022). Cholinergic neuron targeting nanosystem delivering hybrid peptide for combinatorial mitochondrial therapy in Alzheimer’s disease. *ACS Nano, 16*(7), 11455-11472. https://doi.org/10.1021/acsnano.2c05795

8. Navas‐Madroñal, M., Almendra‐Pegueros, R., Puertas-Umbert, L., Jiménez‐Altayó, F., Julve, J., Pérez, B., … & Galán, M. (2023). Targeting mitochondrial stress with szeto‐schiller 31 prevents experimental abdominal aortic aneurysm: crosstalk with endoplasmic reticulum stress. *British Journal of Pharmacology, 180*(17), 2230-2249. https://doi.org/10.1111/bph.16077

9. Sakellariou, G., Pearson, T., Lightfoot, A., Nye, G., Wells, N., Giakoumaki, I., … & McArdle, A. (2016). Mitochondrial ros regulate oxidative damage and mitophagy but not age-related muscle fiber atrophy. *Scientific Reports, 6*(1). https://doi.org/10.1038/srep33944

10. Zhang, L., Feng, M., Wang, X., Zhang, H., Ding, J., Cheng, Z., … & Qian, L. (2021). Peptide szeto‑schiller 31 ameliorates doxorubicin‑induced cardiotoxicity by inhibiting the activation of the p38 mapk signaling pathway. *International Journal of Molecular Medicine, 47*(4). https://doi.org/10.3892/ijmm.2021.4896

 

[GimmeABreak]

I've just started my 3d week of SS31 today. I stopped using lipo-c with NAD+ (Pegasus from Kimera Chems) for a few weeks before starting ss31 because I wanted to feel whatever there might be to feel with ss31, without external energy. And I'm very glad that I did. I plan on doing the three stages of this protocol - ss31/mots-c/Nad+ - in that order - and either for 8 weeks each or 12 weeks each. Though I have no scientific data to point to, I think that using ss31 not only cleans the mitochondria (whether you "feel" anything or not, it's working behind the scenes), but I think it also primes the cells for all the other peps you might research afterwards. I am doing ss31 daily, started low at 1mg, and have moved up to 3mg. I like how I feel - good clean energy and though I'm usually very focused, I do feel like my focus has become even more intense! Though the typical ss31 protocol seems to be 4mg off the bat, daily, running for 21 or 28 days or 1 month or 2 months or more, I wanted to move up slowly and I'm glad I am doing that too - so I started at 1mg, moved to 1.5mg, 2mg, 2.5mg, and now at 3mg over fourteen days. I think I'll stay at 3mg for the whole of this week and then reassess, staying at 3mg or moving up to 3.5mg. Once I decide whether to run ss31 for 8 or 12 weeks, I will add in mots-c about 3 weeks before I finish ss-31. The typical protocol for mots seems to be 5mg 3x a week, but I intend to start low there too, at 1mg again, to see how I react. I'll run mots-c, and increase dosing as feels right to me, drop the ss-31, continue the mots- and either at 8 weeks or 12, I'll introduce the NAD+, and again, I'll start low, not at 25mg with a fast titration schedule up, but at 10mg 3x a week, and move up slowly as I see fit. I can see keeping ss31 as a regular part of my pep protocols.

I have been pinning SS-31 for about 10 weeks now. I also titrated up to 4mg over two weeks. Now I am at 4 or 5mg in the morning and some days I will take an additional 3-4mg in the afternoon. I am planning to stay on it until I no longer receive any benefit from it.

There have a few studies where patients were dosed 40mg per day for almost a year with no side effects beyond the occasional ISR. There was also no titration schedule mentioned in the studies which I thought was odd. I could not imaging 40mg right out of the gate....

I did about a three-week cycle of MOTs-C where I tried it with, and without SS-31 and did not feel anything from MOTs. I still have a few kits so I will probably try adding it back in the next few weeks.

Efficacy and Safety of Elamipretide in Individuals With Primary Mitochondrial Myopathy: The MMPOWER-3 Randomized Clinical Trial - PubMed

This study provides Class I evidence that elamipretide does not improve the 6MWT or fatigue at 24 weeks compared with placebo in patients with primary mitochondrial myopathy.

pubmed.ncbi.nlm.nih.gov

https://www.ophthalmologyscience.org/article/S2666-9145(24)00164-7/fulltext

 

[MingHenry]

More info on ss31 and what you may have experienced (generated with scite.ai):

---

Based on the references provided, the key points about the use of SS31 peptide are:

- SS31 is a mitochondria-targeted antioxidant peptide that can inhibit apoptosis and oxidative stress by maintaining mitochondrial structure and function (1, 2, 3, 4). It has been shown to protect against various conditions involving mitochondrial dysfunction, such as sepsis-induced muscle weakness (2), atrial fibrillation (5), high glucose-induced neuronal damage (3), and tert-butylhydroperoxide-induced oxidative damage in retinal cells (4).

- SS31 has also been used in combination with other drugs or nanoparticles to enhance their therapeutic effects, such as in the treatment of myocardial ischemia-reperfusion injury (1, 6), Alzheimer's disease (7), and abdominal aortic aneurysm (8).

- The protective effects of SS31 have been attributed to its ability to reduce mitochondrial ROS production, stabilize mitochondrial membrane potential, and modulate signaling pathways like the p38 MAPK pathway (9, 10).

In summary, the references indicate that SS31 is a promising mitochondria-targeted antioxidant with a wide range of therapeutic applications, particularly in conditions involving mitochondrial dysfunction and oxidative stress.

References:

1. Zhang, Z., Chen, Z., Yang, L., Zhang, J., Li, Y., Li, C., … & Xiao, W. (2022). Platelet membrane–encapsulated msns loaded with ss31 peptide alleviate myocardial ischemia-reperfusion injury. *Journal of Functional Biomaterials, 13*(4), 181. https://doi.org/10.3390/jfb13040181

2. Supinski, G., Wang, L., Schroder, E., & Callahan, L. (2020). Ss31, a mitochondrially targeted antioxidant, prevents sepsis-induced reductions in diaphragm strength and endurance. *Journal of Applied Physiology, 128*(3), 463-472. https://doi.org/10.1152/japplphysiol.00240.2019

3. Cao, M., Jiang, J., Du, Y., & Yan, P. (2012). Mitochondria-targeted antioxidant attenuates high glucose-induced p38 mapk pathway activation in human neuroblastoma cells. *Molecular Medicine Reports, 5*(4), 929-934. https://doi.org/10.3892/mmr.2012.746

4. Ma, W., Zhu, X., Ding, X., Li, T., Hu, Y., Hu, X., … & Tang, S. (2015). Protective effects of ss31 on t-bhp induced oxidative damage in 661w cells. *Molecular Medicine Reports, 12*(4), 5026-5034. https://doi.org/10.3892/mmr.2015.4055

5. Wiersma, M., Marion, D., Wüst, R., Houtkooper, R., Zhang, D., Groot, N., … & Brundel, B. (2019). Mitochondrial dysfunction underlies cardiomyocyte remodeling in experimental and clinical atrial fibrillation. *Cells, 8*(10), 1202. https://doi.org/10.3390/cells8101202

6. Li, F., Liu, D., Liu, M., Q, J., Zhang, B., Mei, Q., … & Zhou, S. (2022). Tregs biomimetic nanoparticle to reprogram inflammatory and redox microenvironment in infarct tissue to treat myocardial ischemia reperfusion injury in mice. *Journal of Nanobiotechnology, 20*(1). https://doi.org/10.1186/s12951-022-01445-2

7. Qian, K., Bao, X., Li, Y., Wang, P., Guo, Q., Yang, P., … & Zhang, Q. (2022). Cholinergic neuron targeting nanosystem delivering hybrid peptide for combinatorial mitochondrial therapy in Alzheimer’s disease. *ACS Nano, 16*(7), 11455-11472. https://doi.org/10.1021/acsnano.2c05795

8. Navas‐Madroñal, M., Almendra‐Pegueros, R., Puertas-Umbert, L., Jiménez‐Altayó, F., Julve, J., Pérez, B., … & Galán, M. (2023). Targeting mitochondrial stress with szeto‐schiller 31 prevents experimental abdominal aortic aneurysm: crosstalk with endoplasmic reticulum stress. *British Journal of Pharmacology, 180*(17), 2230-2249. https://doi.org/10.1111/bph.16077

9. Sakellariou, G., Pearson, T., Lightfoot, A., Nye, G., Wells, N., Giakoumaki, I., … & McArdle, A. (2016). Mitochondrial ros regulate oxidative damage and mitophagy but not age-related muscle fiber atrophy. *Scientific Reports, 6*(1). https://doi.org/10.1038/srep33944

10. Zhang, L., Feng, M., Wang, X., Zhang, H., Ding, J., Cheng, Z., … & Qian, L. (2021). Peptide szeto‑schiller 31 ameliorates doxorubicin‑induced cardiotoxicity by inhibiting the activation of the p38 mapk signaling pathway. *International Journal of Molecular Medicine, 47*(4). https://doi.org/10.3892/ijmm.2021.4896

I appreciate all this - which I have as well! I think ss31 is up there, for me, with epithalon, kpv, ghk-cu, bpc157, tb4.

I have been pinning SS-31 for about 10 weeks now. I also titrated up to 4mg over two weeks. Now I am at 4 or 5mg in the morning and some days I will take an additional 3-4mg in the afternoon. I am planning to stay on it until I no longer receive any benefit from it.

There have a few studies where patients were dosed 40mg per day for almost a year with no side effects beyond the occasional ISR. There was also no titration schedule mentioned in the studies which I thought was odd. I could not imaging 40mg right out of the gate....

I did about a three-week cycle of MOTs-C where I tried it with, and without SS-31 and did not feel anything from MOTs. I still have a few kits so I will probably try adding it back in the next few weeks.

Efficacy and Safety of Elamipretide in Individuals With Primary Mitochondrial Myopathy: The MMPOWER-3 Randomized Clinical Trial - PubMed

This study provides Class I evidence that elamipretide does not improve the 6MWT or fatigue at 24 weeks compared with placebo in patients with primary mitochondrial myopathy.

pubmed.ncbi.nlm.nih.gov

https://www.ophthalmologyscience.org/article/S2666-9145(24)00164-7/fulltext

Love that you're still on the ss-31 - and titrated up over the 2 weeks, I remember reading how you were doing it, you'd gone up, then dropped back, then went back up. And love that you're feeling great from it obviously - and using up to 9mgs a day?! Also interesting - so many seem to only feel the mots-c and not the ss-31 - but also many only feel the ss31 and get no perceived "feels" from the mots. Look forward to your continued journey. And I am gratified to see you've been doing it for 10 weeks now - because my sense is that a longer cycle is more helpful, and do plan to do a 12 week cycle! QUESTION: at any point titrating up did you find the ss-31 interfered with getting to sleep at all - such as staying up later than intended?

 

[GimmeABreak]

I appreciate all this - which I have as well! I think ss31 is up there, for me, with epithalon, kpv, ghk-cu, bpc157, tb4.

Love that you're still on the ss-31 - and titrated up over the 2 weeks, I remember reading how you were doing it, you'd gone up, then dropped back, then went back up. And love that you're feeling great from it obviously - and using up to 9mgs a day?! Also interesting - so many seem to only feel the mots-c and not the ss-31 - but also many only feel the ss31 and get no perceived "feels" from the mots. Look forward to your continued journey. And I am gratified to see you've been doing it for 10 weeks now - because my sense is that a longer cycle is more helpful, and do plan to do a 12 week cycle! QUESTION: at any point titrating up did you find the ss-31 interfered with getting to sleep at all - such as staying up later than intended?

No issue with sleep at all but I have never taken it about 4:00 in the afternoon. I also only take the second dose like once every week or two. Specifically, if I need a little pick me up because we have evening plans or something. I guess if I pinned at 9PM pin it would probably keep me up. In general, I typically don't have a difficult time falling asleep.

 

[Chuck11]

've been trying SS-31 for a clinically diagnosed mitochondrial disorder, linked to heterozygous PINK1 and OPA1 mutations. I've been taking large doses—15 to 30 mg per day for a week. It's hard to say if it's having a positive effect so far—maybe it would have been more effective if I'd started 15 years ago when the condition became full-blown. The goal is to upregulate mitochondrial fusion (defragmentation) via the working OPA1 copy.

I noticed that clinical trials use massive doses. Does anyone know why SS-31 is so expensive or if there's a way to get it cheaper? I probably need to buy a gram.

At this point, I'm planning to either get somewhat healthier or die trying—no joke. After a decade of promises, a Harvard-trained biomedical geneticist and mitochondrial expert told me there’s nothing in the drug pipeline—nothing panned out—so self-experimentation is my only option.

 

[Vicki]

've been trying SS-31 for a clinically diagnosed mitochondrial disorder, linked to heterozygous PINK1 and OPA1 mutations. I've been taking large doses—15 to 30 mg per day for a week. It's hard to say if it's having a positive effect so far—maybe it would have been more effective if I'd started 15 years ago when the condition became full-blown. The goal is to upregulate mitochondrial fusion (defragmentation) via the working OPA1 copy.

I noticed that clinical trials use massive doses. Does anyone know why SS-31 is so expensive or if there's a way to get it cheaper? I probably need to buy a gram.

At this point, I'm planning to either get somewhat healthier or die trying—no joke. After a decade of promises, a Harvard-trained biomedical geneticist and mitochondrial expert told me there’s nothing in the drug pipeline—nothing panned out—so self-experimentation is my only option.

The best price I've seen was qsc has it on sale for 260 during black Friday and I see it in a group buy now for same price but that's not even going into production until after chiense new year. Both those were 50mg sizes

 

[GimmeABreak]

've been trying SS-31 for a clinically diagnosed mitochondrial disorder, linked to heterozygous PINK1 and OPA1 mutations. I've been taking large doses—15 to 30 mg per day for a week. It's hard to say if it's having a positive effect so far—maybe it would have been more effective if I'd started 15 years ago when the condition became full-blown. The goal is to upregulate mitochondrial fusion (defragmentation) via the working OPA1 copy.

I noticed that clinical trials use massive doses. Does anyone know why SS-31 is so expensive or if there's a way to get it cheaper? I probably need to buy a gram.

At this point, I'm planning to either get somewhat healthier or die trying—no joke. After a decade of promises, a Harvard-trained biomedical geneticist and mitochondrial expert told me there’s nothing in the drug pipeline—nothing panned out—so self-experimentation is my only option.

You're right. If I am remembering correctly the phase 2 and phase 3 trials I reviewed for elamipritide (SS-31) dosed at 40mg/day for like 6-12 months. That's a payment on a nice car (for people that finance cars)!

It's actually getting ready to be an FDA approved med for Barth Syndrome in 1H-2025.

FDA Accepts New Drug Application for Elamipretide | Stealth Biotheraputics

Stealth BioTherapeutics announced that the FDA has accepted for filing its New Drug Application for elamipretide for the treatment of Barth syndrome.

stealthbt.com

 

[Chuck11]

You're right. If I am remembering correctly the phase 2 and phase 3 trials I reviewed for elamipritide (SS-31) dosed at 40mg/day for like 6-12 months. That's a payment on a nice car (for people that finance cars)!

It's actually getting ready to be an FDA approved med for Barth Syndrome in 1H-2025.

https://stealthbt.com/stealth-bioth...ide-for-the-treatment-of-barth-syndrome/[/URL

Also, the route is typically intravenous:

Participants were randomized to intravenous elamipretide (0.01, 0.1, and 0.25 mg/kg/h or placebo for 2 hours in a dose-escalating sequence). The primary efficacy measure was the change in distance walked in the 6-minute walk test (6MWT) after 5 days of treatment. https://www.neurology.org/doi/pdfdirect/10.1212/WNL.0000000000005255

With intramuscular, a good portion will be lost. I was thinking of taking it as an eyedrop. I will try 60mg in one shot next. But at $89 x 2 from Sky Peptides, this is running a pretty penny. Is that has an appreciable effect, I guess I will try to buy a gram for 2.5k https://www.selleckchem.com/products/elamipretide-mtp-131.html

 

[GimmeABreak]

The price at Sellechkchem is $2290 for a gram.

QSC's price mentioned in the previous post is $520 for a gram. Which is two 50mg kits @ $260/kit (a kit is 10 vials). The site you linked also doesn't state if they are selling raws or lyophilized and trust me, you don't want to deal with raws.

 

[GimmeABreak]

With intramuscular, a good portion will be lost. I was thinking of taking it as an eyedrop.

I normally just go SQ, the thought of putting it directly into my eye is a little to cringy for me.

 

[Valerie]

I have had great results with SS-31. Tons of energy and countered the Tirz fatigue.

I pinned 4-5mg in the AM daily for most of October and November after titrating up to to 4mg over about two weeks. Some days I would talso take a 3mg booster dose in the afternoon. I added MOTs-C for about 3-4 weeks but noticed no additional benefits. I am currently taking a week off from peps (except Tirz) but will likely start it back up again next week.

My opinion (with zero medical/scientific training to back this up) based on reading feedback on the forums is if you old and didn't live a healthy lifestyle for most of your life you will get benefit from SS-31 (I'm 58). If you are young or middle-aged and mostly lived clean, it will probably be a nothing-burger for you.

Not sure about the old AND unhealthy lifestyle.

I'm over 50 but have lived a healthy lifestyle.

Mitochondrial function simply declines as we age.

 

[Chuck11]

The price at Sellechkchem is $2290 for a gram.

QSC's price mentioned in the previous post is $520 for a gram. Which is two 50mg kits @ $260/kit (a kit is 10 vials). The site you linked also doesn't state if they are selling raws or lyophilized and trust me, you don't want to deal with raws.

Link to QSC? 1 gram is 1000mg, not 100mg, so I would need 20 50mg kits. As for ocular application, it makes a difference if you have a degenerative condition -- you become more risk-tolerant.

 

[GimmeABreak]

Link to QSC? 1 gram is 1000mg, not 100mg, so I would need 20 50mg kits. As for ocular application, it makes a difference if you have a degenerative condition -- you become more risk-tolerant.

Damn, you had me second-guessing myself. A 50mg kit has 500mg (ten 50mg vials) so two kits = 1000mg = 1g.

Look in the vendor connection forum (on this site), QSC advertises there but only order through Tracy. Search this site to learn more about them, they are not necessarily a vendor for beginners. Also, they are on hiatus probably because of the Chinese New Year or maybe because they were arrested, but reach out to them in early Feb when they are back online. I am sure they will have new promos to kick things back off.

Other CNY vendors stock SS31 but most only stock 30mg (300mg) kits.

Nexaph/SNP is US based and has a preorder right now that will ship in the next week or so, 300mg for $220 with free two-day shipping. They are in the vendor connection channel or you can find them here: https://nexaph.com/product/pre-order-ss-31-30mg-10-vials-kit/

 

[Chuck11]

Damn, you had me second-guessing myself. A 50mg kit has 500mg (ten 50mg vials) so two kits = 1000mg = 1g.

Look in the vendor connection forum (on this site), QSC advertises there but only order through Tracy. Search this site to learn more about them, they are not necessarily a vendor for beginners. Also, they are on hiatus probably because of the Chinese New Year or maybe because they were arrested, but reach out to them in early Feb when they are back online. I am sure they will have new promos to kick things back off.

Other CNY vendors stock SS31 but most only stock 30mg (300mg) kits.

Nexaph/SNP is US based and has a preorder right now that will ship in the next week or so, 300mg for $220 with free two-day shipping. They are in the vendor connection channel or you can find them here: https://nexaph.com/product/pre-order-ss-31-30mg-10-vials-kit/

Thank you so much for the advice! I'll look into it and keep you all updated. This should serve as a canary-in-the-coal-mine test case, as in theory, SS31 upregulates fusion via OPA1.