SS-31 protocols?

[GimmeABreak]

Thank you so much for the advice! I'll look into it and keep you all updated. This should serve as a canary-in-the-coal-mine test case, as in theory, SS31 upregulates fusion via OPA1.

Since you have a legitimate medical diagnosis have you tried getting a Doc to write a prescription to a compound pharmacy and seeing if your health insurance would cover it?

Please keep us posted on your process to create a eye drop and your progress. I would imagine you would want to reconstitute with sterile water or saline? This means the vials become one-time-use as there is no alcohol to prevent bacterial growth. I don't think BAC water in your eye would be very enjoyable.

 

[Chuck11]

Today I tried 60mg from Skype Peptides. The effect wasn't huge but it wasn't nothing. Increased mental clarity. My dystonia was more controlled. I had tried AC peptide before and it did nothing -- probably junk. Will order the 50mg x 10 set and try 100mg for 5 days.

.

 

[Chuck11]

Since you have a legitimate medical diagnosis have you tried getting a Doc to write a prescription to a compound pharmacy and seeing if your health insurance would cover it?

Please keep us posted on your process to create a eye drop and your progress. I would imagine you would want to reconstitute with sterile water or saline? This means the vials become one-time-use as there is no alcohol to prevent bacterial growth. I don't think BAC water in your eye would be very enjoyable.

I see Dr. Fran Kendall, an expert in the field out of Atlanta. Also specialists at Duke. Complex 1,3 defects, seemingly downstream a couple of mutations. They were pessimistic. There were high hopes for Elimipretide but they didn't plan out in the trials during the last 5 years. She said she would prescribe if it ever was approved. I doubt she would write a script for a compounder but I'll ask. I only recently discovered that you could buy as SS31. I was on rapamycin for a while -- with the help of some crazy guys from longecity - and that was a miracle drug via upregulation of mitophagy. In the last 5 years, I was able to travel, due 500 miles of the Camino de Santiago, present research at a small conference, relearn how to drive, write a book, and take care of my dad during his last two years. But homeostasis kicks in along with disease progression. At some point it's like trying to empty the ocean with a teaspoon.

 

[Chuck11]

The best price I've seen was qsc has it on sale for 260 during black Friday and I see it in a group buy now for same price but that's not even going into production until after chiense new year. Both those were 50mg sizes

Where do you see the group buy info? Also, do you guys do group testing?

 

[GimmeABreak]

Where do you see the group buy info? Also, do you guys do group testing?

If you have an immediate need, the Nexaph link I sent is probably going to be the fastest for a kit or two. Cain/Nexaph just got the latest batch in stock today, he takes CC's and ships two-day for free. I have a bunch of SS31 from Cain/Nexaph from a previous batch and I am very happy with it.

QSC or the previously mentioned group buy could be a good second buy for you. You will likely not receive it until mid-late February due to the Chinese new year. If you are going to wait for the group buy testing (assuming its included) it will likely add another month to this. Group buys are great but typically take 6-12 weeks (or longer) to complete, especially if they include 3rd party testing.

Let me know if I can help in any way.

 

[Chuck11]

Ok, thanks. Ordered and recived Nexaph; I did 50mg today in one arm shot. Will let you know if I feel anything.

 

[Chuck11]

Ok, thanks. Ordered and recived Nexaph; I did 50mg today in one arm shot. Will let you know if I feel anything.

The effect with Nexaph SS31 is much more subtle than with Skye SS31. How would I go about getting this third-party tested? I would be willing to pay to test SS31 from various companies so that we all know what we are getting. My condition is not something to monkey around with. And I am not in the mood to piss money down the drain on junk.

 

[GimmeABreak]

The effect with Nexaph SS31 is much more subtle than with Skye SS31. How would I go about getting this third-party tested? I would be willing to pay to test SS31 from various companies so that we all know what we are getting. My condition is not something to monkey around with. And I am not in the mood to piss money down the drain on junk.

The only reliable testing house that I know that can test SS31 is https://janoshik.com/. Jano is the gold standard of testing in the gray market.

You may get additional options from others on the site but I recommend you stay away from Vanguard and Forever Young Pharmacy. I would also not share the mg in the vial you are sending if you send to anyone but Jano. Cain/Nexaph normally sets up 3rd party testing through a coordinator on this Telegram channel. If they have started it yet on this batch you could volunteer to provide a vial and get your test for free. You can find the link on his website (at the bottom of the page).

 

[Fisherman2356]

If you have an immediate need, the Nexaph link I sent is probably going to be the fastest for a kit or two. Cain/Nexaph just got the latest batch in stock today, he takes CC's and ships two-day for free. I have a bunch of SS31 from Cain/Nexaph from a previous batch and I am very happy with it.

QSC or the previously mentioned group buy could be a good second buy for you. You will likely not receive it until mid-late February due to the Chinese new year. If you are going to wait for the group buy testing (assuming its included) it will likely add another month to this. Group buys are great but typically take 6-12 weeks (or longer) to complete, especially if they include 3rd party testing.

Let me know if I can help in any way.

I agree with Nexaph being a very reliable and quick to ship supplier. I've sourced 4-5 different pareptides from them and the overall experience is night and day compared to other Chinese suppliers I've used. As for quality I couldn't independently verify since the effect of many peptides is rather subtle to me and Nexaph are no different, and I have never sent vials for testing, so I guess I'll take their testing/word for it, but that seems the case for most suppliers also. As for price, it is very competitive considering they only charge $5 for shipping, and free I think on orders over $200.

Out of curiosity what is your (anybody's) perception regarding Nexaph's product quality as compared to let say other Chinese suppliers?

 

[chmuse]

I agree with Nexaph being a very reliable and quick to ship supplier. I've sourced 4-5 different pareptides from them and the overall experience is night and day compared to other Chinese suppliers I've used. As for quality I couldn't independently verify since the effect of many peptides is rather subtle to me and Nexaph are no different, and I have never sent vials for testing, so I guess I'll take their testing/word for it, but that seems the case for most suppliers also. As for price, it is very competitive considering they only charge $5 for shipping, and free I think on orders over $200.

Out of curiosity what is your (anybody's) perception regarding Nexaph's product quality as compared to let say other Chinese suppliers?

If you get the chance, look at the threads the vendor has posted in. Lots of info if you read the entire threads.

 

[Fisherman2356]

If you get the chance, look at the threads the vendor has posted in. Lots of info if you read the entire threads.

Ok thanks for the reply, I think I misspoke. my question should have been: Has any group/person ever tested Nexaph's product independently/on their own?

 

[chmuse]

Ok thanks for the reply, I think I misspoke. my question should have been: Has any group/person ever tested Nexaph's product independently/on their own?

Yes, most of his products get tested third party after the fact. There's an entire telegram for it.

 

[lazygardener]

Trial dosages are in the range of 20mg (low dose trials)-40mg/day sub-q. Based on that, there's no reason to think less than 1mg/day is going to do literally anything besides give your an occasional bruise from the injection. Here's an example for dry eye age related macular dysfunction. https://clinicaltrials.gov/study/NCT06373731?intr=Elamipretide&rank=4

This is a peptide where human clinical trial data actually exists. 500mcg is pointless.

 

[rkl123]

Trial dosages are in the range of 20mg (low dose trials)-40mg/day sub-q. Based on that, there's no reason to think less than 1mg/day is going to do literally anything besides give your an occasional bruise from the injection. Here's an example for dry eye age related macular dysfunction. https://clinicaltrials.gov/study/NCT06373731?intr=Elamipretide&rank=4

This is a peptide where human clinical trial data actually exists. 500mcg is pointless.

Placebo effect is a hell of a drug.

 

[Chuck11]

Trial dosages are in the range of 20mg (low dose trials)-40mg/day sub-q. Based on that, there's no reason to think less than 1mg/day is going to do literally anything besides give your an occasional bruise from the injection. Here's an example for dry eye age related macular dysfunction. https://clinicaltrials.gov/study/NCT06373731?intr=Elamipretide&rank=4

This is a peptide where human clinical trial data actually exists. 500mcg is pointless.

40mg/daily was also used for the mito clinical trial. https://pmc.ncbi.nlm.nih.gov/articles/PMC10382259/ Prohibitively expensive at current prices.

 

[Skidude]

After looking at many different options my first cycle was 4mg 5days on 2 days off for 6 weeks.

I am now ready for my next and will be stacking with overlaps as well- SS-31, Mots-C, NAD+

This is my current SS-31 protocol- Going with Basic first. 5mg a day also taking CoQ10,TMG,Lipo-c w/b12

Basic SS-31 Cycle (General Health & Mitochondrial Support) Dosage: 5-10 mg subcutaneous (SQ) daily Duration: 6-8 weeks Break Between Cycles: 6-8 weeks off Considerations: Take on an empty stomach for better mitochondrial uptake. Pair with Lipo-C, B12, or CoQ10 for enhanced mitochondrial function. Combine with low-impact exercise (walking, bodyweight training) for best results. Advanced SS-31 Cycle (Athletic Recovery & Performance) Dosage: 10-20 mg SQ daily Duration: 8-12 weeks Break Between Cycles: Equal to cycle length (8-12 weeks) Additional Support: Lipo-C/B12 injections for metabolism & energy. Creatine, TMG, and CoQ10 for added mitochondrial support. Cold therapy or sauna use to enhance mitochondrial function. Optional: Microdosing Approach (for Longevity & Maintenance) Dosage: 5 mg SQ 3-4x per week Duration: Ongoing with periodic breaks Breaks: 2-4 weeks off every 3 months

 

[Calm Logic]

After looking at many different options my first cycle was 4mg 5days on 2 days off for 6 weeks.

Thanks for the info. More info on your first cycle:

[archived internal link]

[archived internal link]

Post in thread 'Did you ever feel like you knew everyone on a TV show?'

Mar 14, 2025

that's good information thank you.

what do you think of ss31? i have a kit and i'm itching to do it.

It is tough to say, I am hoping it is helping with the mots etc.. not a huge "feel" for me, but i did notice some increased energy, which on 1200 calories is much needed. I also hope it helps with the Mots-c efficacy. I have been taking TMG supplement to help with those as well.

Overall any effects would be positive, and worth the first cycle for sure.. IMHO

Skidude

 

[Skidude]

Thanks for the info. More info on your first cycle:

[archived internal link]

Post in thread 'Did you ever feel like you knew everyone on a TV show?'

Mar 14, 2025

that's good information thank you.

what do you think of ss31? i have a kit and i'm itching to do it.

It is tough to say, I am hoping it is helping with the mots etc.. not a huge "feel" for me, but i did notice some increased energy, which on 1200 calories is much needed. I also hope it helps with the Mots-c efficacy. I have been taking TMG supplement to help with those as well.

Overall any effects would be positive, and worth the first cycle for sure.. IMHO

Skidude

Click to expand...

I am far from an expert on any of this, and most of the time blindly trying to find my way in the dark, but I do believe this is like many other supplements/peps/meds in that it is very specific to individual physiology.

If a person with ADHD takes Adderall it calms them down, it has a very different effect if you do not.

Same with B12 deficiency, and many other related issues.

This makes it hard to pinpoint what is working- And makes all of this anecdotal IMHO.

Elamipretide (SS-31), a synthetic tetrapeptide, is being investigated for its potential to help individuals with damaged mitochondria . SS-31 is designed to selectively target mitochondria, aiming to restore their function, particularly in conditions like mitochondrial diseases and aging.

Maybe my body needed ss-31 to help other peps work, maybe it did not.

Perhaps it is just a motivation to improve that is the real "pep" in my step.

I may never know.

For now, I am happy with the Red pill or the Blue pill, the steak is delicious, albeit much smaller than it used to be.

 

[Calm Logic]

Yeah, I'm mostly interested in the info for a relative with mild/moderate chronic kidney disease. But the ideal, more experimental dosing is very expensive. So I see it more as an add-on to the more affordable BPC-157, which may also help with CKD.

The Mitochondrion: A Promising Target for Kidney Disease - PMC

Mitochondrial dysfunction is important in the pathogenesis of various kidney diseases and the mitochondria potentially serve as therapeutic targets necessitating further investigation. Alterations in mitochondrial biogenesis, imbalance between ...

pmc.ncbi.nlm.nih.gov

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I think what may help the most about this research is finding cheap supplements that would help with similar pathways. Supplements such as MitoQ, CoQ10, Alpha-Lipoic Acid (ALA), N-Acetylcysteine (NAC), L-Carnitine, Resveratrol, Green Tea Polyphenols (EGCG), and Curcumin/Tumeric.

More info on SS-31 for CKD:

SS-31, a mitochondria-targeting peptide, has shown promise in ameliorating kidney disease, including Chronic Kidney Disease (CKD). Several studies suggest it can:

Improve kidney function: By lowering urinary albumin excretion, proteinuria, serum creatinine, and BUN.

Protect against oxidative stress: SS-31 can reduce reactive oxygen species (ROS) production, preventing damage to renal cells.

Reduce inflammation: It can inhibit the production of pro-inflammatory cytokines.

Prevent mitochondrial dysfunction: SS-31 protects mitochondrial structure and function, which is crucial in kidney health.

Protect podocytes: SS-31 has shown protective effects on podocytes, cells vital for kidney function, in diabetic kidney disease models.

However, it's important to note that while preclinical studies and some clinical trials show positive results, further research is needed to confirm these findings and establish the long-term effectiveness and safety of SS-31 for CKD. Some studies also indicate that the effectiveness of SS-31 can vary depending on the specific cause and model of kidney injury.

 

[Peloma]

For now, I am happy with the Red pill or the Blue pill, the steak is delicious, albeit much smaller than it used to be.

I feel ya. I've been on 5mg daily for 11 days. Starting Mots-c on day 14. 5mg daily with two days off (3/2). Ss-31 will probably always be part of the rotation since I have felt some energy and focus gains.