is it normal to lose ~3/4ml when filtering?

[HereKittyKitty]

In fact flush with as much as you want to make sure the peptides come through as much as possible. Then measure the final volume (draw up into a new sterile syringe, you needn’t even pull the needle out of the vial) to calculate your final concentration.

How does that work exactly? Draw the entire vial into a syringe tells the final concentration? Will you explain a bit more, please?

 

[HereKittyKitty]

Here's a good video on filtering.

View: https://www.youtube.com/watch?v=9YBj26vpHs0&feature=youtu.be

 

[Labcat]

How does that work exactly? Draw the entire vial into a syringe tells the final concentration? Will you explain a bit more, please?

Draw the entire volume into a syringe. I like to pull it all inside the barrel so the extra bit in the hub is accounted for. Using the markings on the syringe, figure out how many milliliters you have. For example if your liquid sits between the marks for 1.4ml and 3ml, you have 1.6 ml. Put everything back in the vial. If there’s a bit stuck in the hub, pull back some air from the vial and use it to push the liquid back in. Suck some air out of the vial into the syringe when you remove the needle. (Oxygen breaks down BAC, so give it a little vacuum)

Using the mg from the vial you recon’d (for example, 10.1 because you had the batch tested) you divide 10.1mg/1.6ml, giving you the concentration of your compound. Use a calculator here, left to the student as an exercise ;-)

 

[desinr-gal]

Do the 4mm ones come with a prefilter? When I ordered the 13mm from cobetter I automatically picked the kind with a pre filter, (0.65+0.2μ) but maybe that kind will trap more volume at the end so maybe that wasn’t a good idea, does anyone know?

no its just the filter. I now pre wet with bac water. while I am reconning the let it sit. This seems to help. I then flush after. The Tisch 4mm are a bit tempermental sometimes. and they have blocked or blown out. I am extra gentle now.

 

[Labcat]

no its just the filter. I now pre wet with bac water. while I am reconning the let it sit. This seems to help. I then flush after. The Tisch 4mm are a bit tempermental sometimes. and they have blocked or blown out. I am extra gentle now.

That’s a good technique, I bet it also decrease as much preferential peptide binding to the PES.

Blow out sounds bad. Not blown out onto the table, I hope!

 

[desinr-gal]

That’s a good technique, I bet it also decrease as much preferential peptide binding to the PES.

Blow out sounds bad. Not blown out onto the table, I hope!

no thank goodness, into the vial and or cartridge. Creates enough bubbles to cause foam.

so then, you either suck it all out and try again or risk not doing that.. very frustrating.