is it normal to lose ~3/4ml when filtering?

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tendency

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Just started filtering my peptides and was surprised to see I lose about 3/4 of a ml when doing so due to 'slop' in the filter and needle. Is this normal? I can't imagine people are losing this much each time they filter?
 
yes the solution is sitting in the filter, flush it out with maybe 0,5-1 ml of bac.
 
miss-sanne said:
yes the solution is sitting in the filter, flush it out with maybe 0,5-1 ml of bac.
Ah that's a good idea - I knew there was a nice simple one somewhere in there that I'm too boneheaded to figure out 😆 - thanks!
 
This is why we love quantified overfills. End up with the net you paid for even after losses.
 
tendency said:
Just started filtering my peptides and was surprised to see I lose about 3/4 of a ml when doing so due to 'slop' in the filter and needle. Is this normal? I can't imagine people are losing this much each time they filter?
I mentioned this in my post about filtering but was told it’s not a real thing despite the fact you can literally SEE the peptide in the filter (GHK-Cu is blue). I prime before and flush afterward with bac but there’s no way to tell the exact amount in the filter. Somehow ‘random person’ figured out how to prevent the filter from absorbing, I assume she’s not using regular filters like we are, I’d ask her how she manages.
 
3/4 ml? 75 units? Sounds like a big filter. The following summarizes what I've been able to find on the subject:

You want to use the smallest possible filter as a lot of peptide gets left in the filter when you are done using it. For a few approximate examples, a 4mm filter retains about.07ml of reconstituted peptide, a 13mm retains 4x more than a 4mm at about.28ml and a 33mm filter holds a whopping.65ml.

Realistically for individuals filter reconstituted peptides you should use a 4mm filter if you are filtering less than 4ml of peptide or a 13mm filter if you are using more than that. (Source - Internet Floatilla)

I dilute so injections are not so concentrated that a few units either way doesn't make a difference. Ideally .50ml (50 units) and I try to not go lower than .30ml (30 units). I also pre wet the filter by having a bit extra bac in the barrel after reconning lyophilized vials. I remove the recon needle, pop on the filter and plunge the remainder out.
 
nonyabizznez said:
3/4 ml? 75 units? Sounds like a big filter. The following summarizes what I've been able to find on the subject:

You want to use the smallest possible filter as a lot of peptide gets left in the filter when you are done using it. For a few approximate examples, a 4mm filter retains about.07ml of reconstituted peptide, a 13mm retains 4x more than a 4mm at about.28ml and a 33mm filter holds a whopping.65ml.

Realistically for individuals filter reconstituted peptides you should use a 4mm filter if you are filtering less than 4ml of peptide or a 13mm filter if you are using more than that. (Source - Internet Floatilla)

I dilute so injections are not so concentrated that a few units either way doesn't make a difference. Ideally .50ml (50 units) and I try to not go lower than .30ml (30 units). I also pre wet the filter by having a bit extra bac in the barrel after reconning lyophilized vials. I remove the recon needle, pop on the filter and plunge the remainder out.
That’s exactly what I do for the same reasons. Great minds! 🥹
 
nonyabizznez said:
3/4 ml? 75 units? Sounds like a big filter. The following summarizes what I've been able to find on the subject:

You want to use the smallest possible filter as a lot of peptide gets left in the filter when you are done using it. For a few approximate examples, a 4mm filter retains about.07ml of reconstituted peptide, a 13mm retains 4x more than a 4mm at about.28ml and a 33mm filter holds a whopping.65ml.

Realistically for individuals filter reconstituted peptides you should use a 4mm filter if you are filtering less than 4ml of peptide or a 13mm filter if you are using more than that. (Source - Internet Floatilla)

I dilute so injections are not so concentrated that a few units either way doesn't make a difference. Ideally .50ml (50 units) and I try to not go lower than .30ml (30 units). I also pre wet the filter by having a bit extra bac in the barrel after reconning lyophilized vials. I remove the recon needle, pop on the filter and plunge the remainder out.
Yeah, you're right. Thanks for the heads up I was using too large of a filter 🙂
 
deleted.user.18 said:
This is why we love quantified overfills. End up with the net you paid for even after losses.
But you don't have to lose anything. Just flush with bac water equal to what's trapped in the filter. Voilà!
 
MeedzMoar said:
But you don't have to lose anything. Just flush with back water equal to what's trapped in the filter. Voilà!
By gad you e cracked the code! Shout it from the roof tops!
 
In fact flush with as much as you want to make sure the peptides come through as much as possible. Then measure the final volume (draw up into a new sterile syringe, you needn’t even pull the needle out of the vial) to calculate your final concentration.
 
I'm new and just read the post of filters, I have no idea. Do you have to filter Reta or Tirz, and what is such a filter. Can you get it in a pharmacy. I would have just bac water and the bottle and the next day I would have put it in a syringe. Sorry for the question.
 
Chrissven said:
I'm new and just read the post of filters, I have no idea. Do you have to filter Reta or Tirz, and what is such a filter. Can you get it in a pharmacy. I would have just bac water and the bottle and the next day I would have put it in a syringe. Sorry for the question.
It's a good idea yes to filter out non-wanted impruritites.

i don't think you buy it in a pharmacy.

it's these you can use for the glp-1's.

https://www.aliexpress.com/item/1005008493243482.html?spm=a2g0o.productlist.main.1.7122wvaswvasQm&algo_pvid=81c3250b-e2d3-4fe0-90a9-75340d002794&algo_exp_id=81c3250b-e2d3-4fe0-90a9-75340d002794-0&pdp_ext_f=%7B%22order%22%3A%22181%22%2C%22eval%22%3A%221%22%2C%22fromPage%22%3A%22search%22%7D&pdp_npi=6%40dis%21DKK%21106.96%2164.15%21%21%2116.24%219.74%21%402103891017653511190112290e1f8b%2112000046427959431%21sea%21DK%210%21ABX%211%210%21n_tag%3A-29910%3Bd%3A4b4fd153%3Bm03_new_user%3A-29895%3BpisId%3A5000000187455099&curPageLogUid=xwzppK2ZZTQA&utparam-url=scene%3Asearch%7Cquery_from%3A%7Cx_object_id%3A1005008493243482%7C_p_origin_prod%3A
 
desinr-gal said:
I was quite happy I got all 6ml through the 4mm this time without it blowing or blocking.
Do the 4mm ones come with a prefilter? When I ordered the 13mm from cobetter I automatically picked the kind with a pre filter, (0.65+0.2μ) but maybe that kind will trap more volume at the end so maybe that wasn’t a good idea, does anyone know?
 
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