Testing and splitting vials questions

[ilateapex]

New to Gray and doing a lot of reading and research. Thanks to all that post here.

Testing question: If I purchase multiple kits of one product, one supplier, and one size, say T60 X 4 kits. Do I just send one vial for testing or is it necessary to send one vial per kit? What if I purchase some T60 and T30 kits, same question, how many vials to test?

Splitting: If I get T60 kits but only taking around 5mg dose, is it best to split the vial into two smaller vials during reconstitution to avoid pinning one vial too often? I have this same question for some compounded I am currently taking. I have some 72mg vials and wondering if I should remove half of it and put in a new vial to avoid possible contamination due to excessive pinning.

Thanks for any help.

 

[Grogu]

New to Gray and doing a lot of reading and research. Thanks to all that post here.

Testing question: If I purchase multiple kits of one product, one supplier, and one size, say T60 X 4 kits. Do I just send one vial for testing or is it necessary to send one vial per kit? What if I purchase some T60 and T30 kits, same question, how many vials to test?

Splitting: If I get T60 kits but only taking around 5mg dose, is it best to split the vial into two smaller vials during reconstitution to avoid pinning one vial too often? I have this same question for some compounded I am currently taking. I have some 72mg vials and wondering if I should remove half of it and put in a new vial to avoid possible contamination due to excessive pinning.

Thanks for any help.

If you're doing the testing on your own, I personally would recommend at least 1 vial for mass/purity and one for endotoxins, given the recently high results seen for endotoxins. Depending on your risk tolerance, you might also test for sterility, which depending on the lab could be one or two vials. So, at a minimum, you probably want to do 2 vials (mass/purity and endotoxins). And this would be for each production batch. So, separate testing for your T60 kits versus T30. I'd personally would be more focused on testing those T30 kits. You might also look for testing groups where you can share in the cost with others for the batches you are pruchasing.

As far as vial punctures and sterility/degradation, I've used compounded vials of tirzepatide for numerous punctures over many months. Check the vial before each injection for any cloudiness or floaters and clearly if there are any visible signs of an issue, don't inject. The "official" medical guidance is 28 days for multi-dose vials, but that's very conservative.

I read one thing really early on in my journey is that diabetics may inject numerous times per day from the same vial that they use over a month. So, a diabetic injecting 3x a day is looking at 90 punctures in a month. It's the same vial system used in all these vials. Excessive pinning isn't the "problem" you need to be worried about. It's sterility and degradation. Turns out (lucky for us) that tirzepatide is a pretty hardy peptide and can withstand a lot. So, keeping open vials for months and months results is very slow degradation. As far as sterility, my thought has been splitting in two separate vials is not a terrible idea, especially if you're on a low dose and are filtering. Definitely filter.

Good luck.

 

[ilateapex]

Thanks for the input.

Another testing question. When you test say a T60 and the test shows it actually has 63 mg, do you assume they all are over filled? So then reconstitute accordingly?

 

[BNLFL]

Thanks for the input.

Another testing question. When you test say a T60 and the test shows it actually has 63 mg, do you assume they all are over filled? So then reconstitute accordingly?

Yes.

 

[Grogu]

Thanks for the input.

Another testing question. When you test say a T60 and the test shows it actually has 63 mg, do you assume they all are over filled? So then reconstitute accordingly?

People do it both ways. A matter of personal preference really. Some just use the stated milligrams and dose based on that and some people adjust to the tested amount. But, with only testing one vial, you really don't have enough data to make any assumptions about the population.

Mass/purity will at least tell you that the vial you sent is the peptide that it claims to be and if there is any significant underfill or overfill. I personally just dose based on the stated milligrams and any overfill is like a bonus both figurately and physically.

Since you're on 5mg that would be 12 doses. If you base it on 12 doses, then the 3 extra milligrams would only be 3mg/12 = .25mg more than expected.

 

[BNLFL]

People do it both ways. A matter of personal preference really. Some just use the stated milligrams and dose based on that and some people adjust to the tested amount. But, with only testing one vial, you really don't have enough data to make any assumptions about the population.

Mass/purity will at least tell you that the vial you sent is the peptide that it claims to be and if there is any significant underfill or overfill. I personally just dose based on the stated milligrams and any overfill is like a bonus both figurately and physically.

Since you're on 5mg that would be 12 doses. If you base it on 12 doses, then the 3 extra milligrams would only be 3mg/12 = .25mg more than expected.

I have J5 R60 that is tested at 72mg, and some R50 at 56mg. When I recon mix it will be 3ml BAC and just adjust the shot amount. I might try his R50 next. I still have 3 or 4 pins left on the current vials.

 

[ilateapex]

Thanks for the replies. This helps a lot.

 

[Grogu]

I have J5 R60 that is tested at 72mg, and some R50 at 56mg. When I recon mix it will be 3ml BAC and just adjust the shot amount. I might try his R50 next. I still have 3 or 4 pins left on the current vials.

Personally like to reconstitute to achieve a certain concentration (e.g. 10mg/mL, 20mg/mL, etc.) to make dosing easier, especially if I want to titrate frequently or change my dose on a whim so I can do the math in my head. But that's the great thing about grey, there is a lot of flexibility.

 

[giraffonaut]

I have J5 R60 that is tested at 72mg, and some R50 at 56mg. When I recon mix it will be 3ml BAC and just adjust the shot amount. I might try his R50 next. I still have 3 or 4 pins left on the current vials.

Slightly off topic, but I've heard them mentioned a number of times and they seem reliable, but I haven't been able to track down any form of contact. I'm guessing they're someone I'm required to be more "in the know" or know the right people before I'd even be about to think about ordering from them. Is that correct?

 

[Estarossa19]

Slightly off topic, but I've heard them mentioned a number of times and they seem reliable, but I haven't been able to track down any form of contact. I'm guessing they're someone I'm required to be more "in the know" or know the right people before I'd even be about to think about ordering from them. Is that correct?

Unfortunately, you have a No Source Discussion tag as you're a newer member. Keep participating in forum conversations and you'll have that removed in no time!

 

[giraffonaut]

Unfortunately, you have a No Source Discussion tag as you're a newer member. Keep participating in forum conversations and you'll have that removed in no time!

Ah my b. Was thinking that was me giving opinions on them not just asking.

 

[BNLFL]

Slightly off topic, but I've heard them mentioned a number of times and they seem reliable, but I haven't been able to track down any form of contact. I'm guessing they're someone I'm required to be more "in the know" or know the right people before I'd even be about to think about ordering from them. Is that correct?

They are hard to find, and you now need to take a test to join. You'll get there.

 

[ilateapex]

Continuing with the splitting vials question.

Upon researching here I can only find BAC in 30ml vials. Research also states that 90 days +/- is the shelf life after first pin. I am assuming an average of 5mg of product needed per week long term therefore T60 would last about 12 weeks or 90 days. I was planing on just reconstituting one 60mg vial when needed. Even splitting that into two vials. So at this pace I would only be using about 2-3 ml of BAC per 90 days. I could technically get a second 60mg reconstituted within the 90 days but that is about it. That would bring it to 4-6 ml per 90 days. This was my plan to keep the product long term in the freezer and the reconstituted product in the fridge.

The 90 days shelf life after pin of the BAC has made me wonder about this approach. Am I thinking about this right? I don't plan on obtaining any additional Peptides and just plan on single use of T.

What does the wisdom here say? Any smaller quantity vials of quality BAC? Do I just build the cost of the waste into my plan?

 

[BNLFL]

Upon researching here I can only find BAC in 30ml vials.

I and others use it till it's almost gone.

 

[ilateapex]

When using BAC for an extended time do you just look for the same visual cues as for the other peptides? Floaters and cloudy?

 

[MG9550]

When using BAC for an extended time do you just look for the same visual cues as for the other peptides? Floaters and cloudy?

You should always inspect the BAC water before use but I make sure mine is all used up within 90 days of first puncture.