Questions About Sterile Empty Vials

[AndyPanda]

I’m not understanding how one filled up with water. Autoclave is supposed to be dry.

 

[stickfigure]

There was some steam condensation on the bottom of the container. I guess there was enough condensation to fill the vial with popped plunger.

The manual for autoclave calls for releasing pressure at the end of the cycle. Next time I'll try to let it cool off naturally before opening the release valve - I am wondering if rapid pressure drop is making heated air inside of cartridges push out the plungers.

This is the model I have

https://a.co/d/ePXFauF

 

[moonpies4misfits]

I’m not understanding how one filled up with water. Autoclave is supposed to be dry.

Huh? Autoclaves work via steam.

 

[AndyPanda]

D

Huh? Autoclaves work via steam.

Do they not finish by completely drying via a dry cycle?

 

[moonpies4misfits]

D

Do they not finish by completely drying via a dry cycle?

No, you're right. I thought you were saying the process is dry. I don't know how'd you achieve that using a pressure cooker though. I haven't looked into the DIY process for that. I've got a friend who is a professional piercer so if I ever need it, he'd most likely let me use his autoclave.

 

[stickfigure]

I've discarded the one with the water. I guess my biggest concern are the popped plungers. For the experiment I've placed one of the cartridges in an oven, and it also popped the plunger, supporting the theory that this happens because of the expanding heated gas inside of a cartridge.

Is anyone using a process that's not a UV steriliazation? Do the plungers pop for you? What is the temperature envelope? How fast is the autoclave heating and cooling?

My other concern is that pressure cooker autoclave continuously releases pressure in bursts, causing repeated drops in vessel pressure while it operates. I am afraid that this cycling alone could be sufficient to pop the plungers

 

[scarywood75]

I've discarded the one with the water. I guess my biggest concern are the popped plungers. For the experiment I've placed one of the cartridges in an oven, and it also popped the plunger, supporting the theory that this happens because of the expanding heated gas inside of a cartridge.

Is anyone using a process that's not a UV steriliazation? Do the plungers pop for you? What is the temperature envelope? How fast is the autoclave heating and cooling?

My other concern is that pressure cooker autoclave continuously releases pressure in bursts, causing repeated drops in vessel pressure while it operates. I am afraid that this cycling alone could be sufficient to pop the plungers

I have to be careful when filling them just not to pop the plunger so I'd imagine they pop very easily under pressure

 

[stickfigure]

They don't pop if you fill them with pressure relief needle. I am using syringe filter, so filling is inherently slow

But there is nothing to relieve pressure when sterilizing with heat and steam

 

[Bronco]

What are the main benefits in purchasing sterile empty vials? To reconstitute a spicy peptide with more bac water? Make your own glow formula?

 

[nccane]

What are the main benefits in purchasing sterile empty vials? To reconstitute a spicy peptide with more bac water? Make your own glow formula?

Filtering

 

[Dee X]

Huh? Autoclaves work via steam.

Not according to Amazon listings lol. They call dry headvt sterilizers as autoclaves too.

 

[moonpies4misfits]

Not according to Amazon listings lol. They call dry headvt sterilizers as autoclaves too.

Ahh, gotta love the accuracy of amazon listings that use multiple terms that are kinda sorta in the same ballpark as the item in their titles