Injectable Buffered NAD500 – Recon, Storage, IM vs SubQ, & Dosage?

[birdwhacker]

Now I'm worried about the 1000mg vials I have on the way. Sounds like there's no way I'll be able to work my way through the first vial on a starter dose within a brief shelf life. Guess my wife and pets are the lucky benefactors.

Lol you'll be fine man. Read the thread. It's probably just an old wives tale.

The better question is how are you going to get the big doses that are called for, because that shit is spicy. Reconstituting 500mg vials with 3ml burns quite a bit with the stuff I got. 150mg would be 90 units.

And the even better question, will it do any good for you

Seems I bought it just as it went out of fashion.

 

[ftv10hb]

Final update:

No issues reconstituting or storing. I haven't been refrigerating it.

Was doing 50mg every other day, now 50mg a day, for a week or so now.

No improvement to baseline energy at all. In-fact, I feel a little bit tired after injecting but I find that if I power through and work out during this window I feel fine.

Today, I did combine it with 3mg of MotsC (first time trying it) and I feel like they synergized pretty well and I was able to get a lot more out of my cardio than usual. Might be worth it to stack with SS31 & MotsC

Sleep quality may have improved marginally. I think I wake up less and sleep deeper.

Overall not worth it, but I'm gonna continue through the kid. Oral NMN/NR with TMG and niacin flushes every now and then seem to be the way to go. I suspect that people who get a lot out of this stuff are overmethylators or haven't supplemented to boost their natural NAD levels.

NAD+ is one of the most sensitive ones out there after reconstitution. It comes in the amber vials because of how sensitive to light it is, and begins to degrade immediately at room temperature. If you haven’t been refrigerating it, it lost its potency a long time ago. I keep mine in the dark in the fridge, 100mg doses 3x/week, definitely notice the clean energy and general feeling of being capable/productive throughout the day. Pin in the AM only, no effect on sleep either way that I can tell.

Other factor with efficacy of NAD+ is age. We basically have a pool of NAD+ in our bodies that begins to leak and diminish with age. Dosing can replenish that pool but not exceed it. 40+ year olds that have a time or lifestyle-diminished pool will see a more noticeable difference when they supplement NAD+, the younger you are from that the higher your NAD+ levels are naturally and so the less noticeable the difference will be when you supplement.

 

[birdwhacker]

NAD+ is one of the most sensitive ones out there after reconstitution. It comes in the amber vials because of how sensitive to light it is, and begins to degrade immediately at room temperature. If you haven’t been refrigerating it, it lost its potency a long time ago.

Where did you learn this? Mine didn't come in amber vials. By what mechanism does it lose its potency? I can feel it pretty distinctly within minutes of the injection, certainly doesn't feel weaker after a week in the cabinet.

Otherwise, I'm glad that it works for you. But I'll stick to my guns and say it doesn't seem worth the trouble given the price and availability of oral precursors.

 

[ftv10hb]

Where did you learn this? Mine didn't come in amber vials. By what mechanism does it lose its potency? I can feel it pretty distinctly within minutes of the injection, certainly doesn't feel weaker after a week in the cabinet.

Otherwise, I'm glad that it works for you. But I'll stick to my guns and say it doesn't seem worth the trouble given the price and availability of oral precursors.

If it didn’t come in amber vials you honestly should have a word with your vendor, i have never heard of it NOT coming in amber vials and the light exposure would have killed it off long before the room temperature did. Results vary for sure and wouldn’t argue with your decision to continue or not one way or the other, but if someone sold you NAD+ in clear vials and didn’t tell you to wrap the vials in foil or something you have legitimate cause to be upset with them because that is definitely standard.

 

[ftv10hb]

Where did you learn this? Mine didn't come in amber vials. By what mechanism does it lose its potency? I can feel it pretty distinctly within minutes of the injection, certainly doesn't feel weaker after a week in the cabinet.

Otherwise, I'm glad that it works for you. But I'll stick to my guns and say it doesn't seem worth the trouble given the price and availability of oral precursors.

If it was largely in the cabinet in constant dark that will have helped, but between the clear vials and the room temperature you definitely lost significant potency. If you’re going to continue anyway my suggestion would be to wrap the vials in foil after reconstitution and keep in the fridge, and see if you notice a difference over the next vial or not, maybe that will help some

 

[birdwhacker]

If you’re going to continue anyway my suggestion would be to wrap the vials in foil after reconstitution and keep in the fridge

I'm gonna be honest, I don't believe a word of this. Nothing against you at all lol, in this business everyone has their ritual they swear by. But thank you regardless, I'll file this away for later and I may try the foil thing just for the hell of it.

Is your NAD spicy? I don't have test strips or anything, it was advertised as buffered, but it burns a fair bit. I understand that the ph shouldn't go above 6, but I don't have a reference for how that should feel under the skin.

Have you ever tried IM?

 

[ftv10hb]

No offense taken, I think if you ask around more or look into it further you will come across the same info tho. Mine is also not spicy at all, and I only do subQ, nothing against IM, just personally was a leap for me to inject myself at all when I started and IM is another step further that I’m just not motivated to take yet lol.

 

[birdwhacker]

No offense taken, I think if you ask around more or look into it further you will come across the same info tho.

Ah, mine is definitely unbuffered then. Figures. Good thing I'm young and my skin can handle it lol.

I have no doubts that I can come across the info, but I do have doubts that it's true in a meaningful sense.

I just blasted 300mg out of curiosity, on top of the 50mg I had earlier in the day, and I can assure you there's been no loss of potency. I can definitely feel it "working," lol, in this case making me feel a tad uneasy.

 

[fremt52958]

I just received my NAD 1000, from a great place. Definitely an amber vial. I have read several dosing recommendations about doing 3ml BAC to the 1000mg and starting at 15units aka 50mg.

Thoughts?

 

[birdwhacker]

I just received my NAD 1000, from a great place. Definitely an amber vial. I have read several dosing recommendations about doing 3ml BAC to the 1000mg and starting at 15units aka 50mg.

Thoughts?

That's what I did, pretty much. 50mg might be a little strong so plan to sit or lay down right after you inject. Make sure you support your methylation pathways afterwards with some tmg/methyl B.

 

[tendency]

...

Other factor with efficacy of NAD+ is age. We basically have a pool of NAD+ in our bodies that begins to leak and diminish with age. Dosing can replenish that pool but not exceed it. 40+ year olds that have a time or lifestyle-diminished pool will see a more noticeable difference when they supplement NAD+, the younger you are from that the higher your NAD+ levels are naturally and so the less noticeable the difference will be when you supplement.

Ehhh, we have a 'pool' of NAD+ that 'leaks' with age? You can then replenish this 'pool'? I'd be very curious to read the studies that settled these assertions scientifically.

 

[tendency]

If it didn’t come in amber vials you honestly should have a word with your vendor, i have never heard of it NOT coming in amber vials and the light exposure would have killed it off long before the room temperature did. Results vary for sure and wouldn’t argue with your decision to continue or not one way or the other, but if someone sold you NAD+ in clear vials and didn’t tell you to wrap the vials in foil or something you have legitimate cause to be upset with them because that is definitely standard.

Yeah, picked up a kit recently from Amino Lair and it was all non-amber vials - first I've seen this happen. Don't think I'll be a repeat customer.

 

[fremt52958]

Yeah, picked up a kit recently from Amino Lair and it was all non-amber vials - first I've seen this happen. Don't think I'll be a repeat customer.

That’s odd my AL was in an amber vial. Just received it yesterday.

 

[birdwhacker]

Yeah, picked up a kit recently from Amino Lair and it was all non-amber vials - first I've seen this happen. Don't think I'll be a repeat customer.

Mine was Amino Lair too.

To be honest, the amber vials light sensitivity thing is clearly nonsense. The product works fine.

I'm more concerned with the fact that the NAD is un-buffered and advertised as buffered. That's directly harmful.

 

[ftv10hb]

Ehhh, we have a 'pool' of NAD+ that 'leaks' with age? You can then replenish this 'pool'? I'd be very curious to read the studies that settled these assertions scientifically.

My poor attempt at describing a visual that makes sense in my head as far as us having NAD stores in our bodies that get depleted over time as natural processes consume more and more of the NAD with out using or replacing it as efficiently as it did when we were younger lol. In my defense tho, “pools” is a term actually used in NAD research haha

Age-related NAD+ decline - PMC

Nicotinamide adenine dinucleotide (NAD+) is an essential metabolite that is reported to decline in concentration in tissues of aged animals. Strategies to increase NAD+ availability have shown promise in treating many conditions in rodents, ...

pmc.ncbi.nlm.nih.gov

 

[ftv10hb]

Mine was Amino Lair too.

To be honest, the amber vials light sensitivity thing is clearly nonsense. The product works fine.

I'm more concerned with the fact that the NAD is un-buffered and advertised as buffered. That's directly harmful.

There were a lot of links here I didn’t want to go through individually, and I certainly don’t claim to understand all the terminology or mechanism at play myself haha, but fwiw imo there’s enough here for me to believe that NAD+ does degrade significantly when exposed to light and/or left at room temperature. Looking at the symptoms of using degraded NAD+ including stinging and temporary fatigue could also explain your experiences with those as opposed to it being unbuffered. But again I am absolutely not any kind of expert, just wanted to at least share the information that convinced me and directs my storage and use of it personally.

 

[ftv10hb]

Yeah, picked up a kit recently from Amino Lair and it was all non-amber vials - first I've seen this happen. Don't think I'll be a repeat customer.

Yea that is strange, I’ve never ordered there but it seems like they’ve been around long enough to know what the standard is or what’s expected. Even stranger that poster below you received in amber vials just recently too, can’t see a reason for same vendor doing it both ways, especially without any explanation or heads up to the customer. Up to you of course but personally I think that justifies refund/replacement if you wanted it.

 

[tendency]

There were a lot of links here I didn’t want to go through individually, and I certainly don’t claim to understand all the terminology or mechanism at play myself haha, but fwiw imo there’s enough here for me to believe that NAD+ does degrade significantly when exposed to light and/or left at room temperature. Looking at the symptoms of using degraded NAD+ including stinging and temporary fatigue could also explain your experiences with those as opposed to it being unbuffered. But again I am absolutely not any kind of expert, just wanted to at least share the information that convinced me and directs my storage and use of it personally.

There was a forum member on here recently that posted an interesting study, darn if I can find it now, however they, or someone they knew? reconned a vial of NAD+ then left it in the fridge. After 15 days they had the NAD+ levels tested by a lab and it came back 50% degraded. So, yes, I agree that once reconned NAD+ has a fairly short shelf life.

I'm not sure, however, how sensitive the lyophilized un-reconned NAD+ powder is. That may very well be less sensitive to degradation. Sure would like to know definitively.

 

[ftv10hb]

There was a forum member on here recently that posted an interesting study, darn if I can find it now, however they, or someone they knew? reconned a vial of NAD+ then left it in the fridge. After 15 days they had the NAD+ levels tested by a lab and it came back 50% degraded. So, yes, I agree that once reconned NAD+ has a fairly short shelf life.

I'm not sure, however, how sensitive the lyophilized un-reconned NAD+ powder is. That may very well be less sensitive to degradation. Sure would like to know definitively.

Yea, I would too, especially considering how much of the powder I have sitting in the fridge right now lol. And if that’s what happens to it reconstituted even in the fridge I should probably switch to the 500mg vials instead of the 1000 lol

 

[Loveandpeaceandjoy]

PGB is one of the top and most recommended suppliers of buffered NAD. They always have clear vials so not sure if folks are really concerned about the amber vials. Have used them exclusively for NAD over the last year and haven’t noticed a difference with effectiveness at 53 yo. 500 mg vial, sub q, refrigerated, typically lasts about a month.