how to NAD+

[tendency]

I'm not sure anyone answered your buffering question. A while back NAD+ was reconstituting so acidic that you were basically injecting an acid, which cause serious injection site reactions, if not worse. "Buffering" was supposed to be a more stable and useable PH.

In my experience, even the ones that claimed to be buffered were still acidic. There are big threads on what is the optimum PH. Here is what Gemini AI says:

"While NAD+ is most stable at lower pH levels, the human body's physiological pH is approximately 7.4 .

Irritation Threshold: Injections with a pH below 3.0 or above 9.0 typically cause significant pain, burning, and tissue irritation at the site of administration.

Injection Site Comfort: A pH of 5.0 to 6.0 is generally tolerable for subcutaneous or intramuscular injections, providing a balance that preserves the potency of the NAD+ for several weeks (when refrigerated) without causing severe discomfort."

Unfortunately, I have had to test and adjust all of the batches of NAD+ that I have received, even if they claim to be buffered. Buy some PH strips off of Amazon. They are cheap. Buy some injectible Sodium Bicarbonate (for god's sake, not baking soda). And then, it is just trial and error. Different people have different methods. If I'm looking to end up with 5ml in a 500mg vial, I will add 3ml of BAC, and then slowly add sodium bicarbonate and keep testing for PH until it gets in the optimum range. Then, having kept track of how much SB I added, I add the rest of the BAC water and do one final test for PH. I can get it in the range, but the process is not precise. Still, better than injecting acid.

Personally I've never had any issues injection wise with any of the buffered NAD+ I've tried from several vendors. 0 ISR from all of them.

 

[Calm Logic]

Personally I've never had any issues injection wise with any of the buffered NAD+ I've tried from several vendors. 0 ISR from all of them.

But from your old posts, it wasn't much help either subq? So you took by IM instead? Do you prefer oral supplements now?

Just curious since I just started NAD+ today. It stinged somewhat going in. Even though it was a starting dose of 25 mg (and sold as buffered), it gave me an uncomfortable ISR for an hour or so.

I had diluted the 500-mg vial of NAD+ with 3 mL Hospira, but I will add more Hospira (another 3 mL) and am considering IM:

I think the consensus from what I've read is most do it SubQ, I'm a bit of an outlier doing IM.

I resorted to IM for gluta to avoid ISRs, and a clinic here seems to do gluta and NAD+ by IM (in addition to IV).

 

[tendency]

But from your old posts, it wasn't much help either subq? So you took by IM instead? Do you prefer oral supplements now?

Just curious since I just started NAD+ today. It stinged somewhat going in. Even though it was a starting dose of 25 mg (and sold as buffered), it gave me an uncomfortable ISR for an hour or so.

I had diluted the 500-mg vial of NAD+ with 3 mL Hospira, but I will add more Hospira (another 3 mL) and am considering IM:

I resorted to IM for gluta to avoid ISRs, and a clinic here seems to do gluta and NAD+ by IM (in addition to IV).

IM does seem to work better for me than subq. Also, the longer I've been on it the more benefits I'm noticing. I take 50mg daily.

Never made any sense, based upon what I know regarding NAD, to take it once or twice per week or dose NAD+ per the mitochondrial rejuvenation protocol you see on here often. The folks that research the NMN/NR precursors are pretty adamant they need to be taken daily and for multiple months to see the full benefits. I don't see why NAD+ injections would be any different.

The NMN/NR orals never did much for me either for whatever reason. I've always diluted my 500mg NAD with 3ml of hospira as well.

 

[tendency]

Actually I was mistaken; I typically recon 500mg of NAD+ with 5ml of hospira, so, maybe that's helping the ISR.

 

[miameow]

NAD+ dosing seems to be all over the place - not knowing your age or history it's difficult to say so definitely follow a conservative approach titrating up as posted here.

As for myself, I started from 100mg daily and am still there, but I am old and have been experimenting with the precursors for many years before NAD+ injections became a thing. I still have nicotinamide ampoules ready to go as I prefer it over NAD+ sometimes. Just 10mg gives me an instant boost, which sadly doesn't last very long.

In terms of being NAD being buffered.. I've found that even the "buffered" ones sting a lot and I presume this is just label appeal and most of them are unbuffered. I'm too lazy to test the pH and have sodium chloride and sodium bicarb solutions on hand, which I no longer use as I've become used to the sting.

Unfortunately despite being old and low on energy I have not seen much benefit from NAD. I am wondering if I should go up even higher or switch to oral. Maybe I need mots. But for the time being I am pinning away as I have a lot to go through.

 

[domin8brix]

I tried NAD for like 4ish months and felt ZERO effects. I would feel that weird warm "rush" after the injection, but that was it. No energy, no added weight loss, no nothin! I am wondering if I was not dosing it right, since it seems like it should be going UP vs a front load and then going "down", so I might mix up a fresh vial and try again using protocols I've seen around here.

 

[PAPoots]

I did the NAD+, MOTS-c, and SS-31 protocol that is floating around in various thread. There is probably a copy of it in this thread somewhere. But I got the best benefits from MOTS-c and NAD+ towards the end of the protocol. It felt like taking pre-workout, but without the fast heart rate caffeine causes. Post Mots-c the Nad+ still hit decent on its own for about 2 months, but the effect has been dwindling. So, I might run the protocol again in another month or so. Possibly after running an Epitalon protocol.

 

[PAPoots]

So, I was looking at Amazon Haul last night and there are a ton of various NAD+ oral supplements available. Usually mixed with other longevity supps, like CoQ10, Resveratrol and a few others. Has anyone taken these? And does the NAD+ work anywhere as near as well as taking it SubQ?

 

[Calm Logic]

I tried NAD for like 4ish months and felt ZERO effects.

Lots of people feel the same way with most of the non-GLP peptides.

The biggest problem with the non-GLPs is they distract from hoarding GLPs, haha.

 

[Tcah2456]

I did the NAD+ with the full MITO mix: ss-31/MOTSc/NAD+

I did 100 mg MWF

for the term: see below

View attachment 19222

I did take TMG to replenish Methyl

Practical Recommendations for NAD+ & TMG Supplementation ​

Timing and Dosage

Based on current knowledge, TMG’s role as a long-acting methyl donor means it can be taken at any time of the day . There’s no evidence to suggest that TMG must be taken at the same time as NAD+. A typical dosage is 1,000mg of TMG daily , which should be more than enough to support methylation needs. However, for individuals who find daily TMG supplementation burdensome, taking it only on the days they use their NAD+ patch is also a viable option.

Daily vs. Occasional Supplementation

While some patients prefer to take TMG daily, others opt to only take it when using their NAD+ patches. Both methods are acceptable, and individual responses can vary based on personal methylation capacity.

Methylation Testing

Unfortunately, there aren’t yet precise tests to measure methylation efficiency or NAD+ metabolism in a way that would inform personalized TMG dosing. However, genetic testing for MTHFR mutations and measuring homocysteine levels can provide insight into your methylation capacity.

The Takeaway ​
While the precise timing and the amount of TMG patients should use with NAD+ are not set in stone, our approach is based on what seems to work for most people: either take TMG daily or on the days when you use NAD+ supplements, especially if you have known methylation issues. You can easily find TMG supplements at online retailers or most vitamin and supplement shops.

At Sellers website, we’ll continue to monitor the latest research and provide updated guidance as more is understood about the interplay between NAD+ and methyl donors like TMG.

Taking Trimethylglycine (TMG) alongside NAD+ precursors (like NMN or NR) helps replenish methyl groups, which are depleted during the metabolic process of raising NAD+ levels. This combination supports optimal methylation , crucial for DNA repair, detoxification, and preventing high homocysteine, often taken together in a 1:1 ratio.

Key Aspects of Combining TMG with NAD+ Boosters:

Why Combine Them: NAD+ boosters help energy production but can drain the body’s methyl group supply. TMG is a "methyl donor," restoring these groups to keep methylation pathways open.

Optimal Ratio & Dosage: Experts, including Dr. David Sinclair, often recommend a 1:1 ratio of TMG to NMN/NR, commonly around

View attachment 19219 When to Take: TMG can be taken in the morning, often with food to maximize absorption and prevent stomach discomfort.

View attachment 19221

of TMG daily.

Synergy: While NMN/NR directly raises NAD+, TMG ensures the metabolic process does not cause long-term depletion of methyl groups, supporting, overall, cellular health.

Benefits: Potential benefits include sustained energy, improved cardiovascular health, and better methylation, with significant improvements often reported after 3-6 months.

Great info I found 15mg motc a week was great on its own so this must be dynamite from reading the potential synergistic effects, could you tell me how you got on with the protocol any sides/ benefits?

Thank you

 

[PAPoots]

I tried NAD for like 4ish months and felt ZERO effects. I would feel that weird warm "rush" after the injection, but that was it. No energy, no added weight loss, no nothin! I am wondering if I was not dosing it right, since it seems like it should be going UP vs a front load and then going "down", so I might mix up a fresh vial and try again using protocols I've seen around here.

Did you workout after taking the NAD+? Expecting weight loss? I really never thought of it as a weight loss compund.

To me, NAD+ was like taking creatine. You won't know it's working until you put it to work. My reps went up a lot, so much so that I was making better gains and could increase the weight at the next workout.

I'm actually on the site today looking for promotions. I want to run another SS-31/MOTS-c, NAD+ protocol before summer.

 

[PAPoots]

Doing a bit more research, maybe it was the MOTS-c helping me. Either way, I think you should do the protocol for the synergistic effects.

Goal MOTS‑c NAD+ More reps Strong effect Mild Delay fatigue Strong Mild Increase lactate threshold Strong None Improve mitochondrial efficiency Strong Moderate Improve recovery Moderate Strong Improve daily energy Moderate Strong Fat‑loss synergy Strong Mild

 

[domin8brix]

Did you workout after taking the NAD+? Expecting weight loss? I really never thought of it as a weight loss compund.

To me, NAD+ was like taking creatine. You won't know it's working until you put it to work. My reps went up a lot, so much so that I was making better gains and could increase the weight at the next workout.

I'm actually on the site today looking for promotions. I want to run another SS-31/MOTS-c, NAD+ protocol before summer.

I took it three times a week, but usually not near a workout, a few hours after or maybe like 8 hours before. I did it when I pinned, and my original tirz pin was Friday 8am, Sunday 2pm, Tuesday 8pm. But I literally felt NOTHING, though same with Creatine. That didn't do much either except send my liver enzymes to near fatal levels.

NAD was sold to me as "helps burn more fat and give more energy" but none of that appears to have been the case for me

 

[PAPoots]

I took it three times a week, but usually not near a workout, a few hours after or maybe like 8 hours before. I did it when I pinned, and my original tirz pin was Friday 8am, Sunday 2pm, Tuesday 8pm. But I literally felt NOTHING, though same with Creatine. That didn't do much either except send my liver enzymes to near fatal levels.

NAD was sold to me as "helps burn more fat and give more energy" but none of that appears to have been the case for me

How much creatine were you taking? I only take the standard 5mg a day. I'd guess that I miss that dose a few days a week to.

Also, how old are you? most of the mitochondria hacks are for older people whose mitochondria are in disrepair.

I've found the best hack for energy is sleep. Are your sleep scores in the good to excellent range? If you feel the need for a nap during the day, I'm guessing you aren't sleeping well.

If your sleep is good and you don't have energy, or you have brain fog, get your blood work done. I tried a lot of the peptides that were supposedly good for sleep and none of them helped.

My last blood test showed me having an iron deficiency (Ferritin 7.8 ng/mL) and also low B12 (327 pg/mL). I started taking an Iron supp a few times a week, B12 daily, Magnesium glycinate and L-theanine nightly, pushed through the day without taking a nap and made sure I got at least 7-9 hours of sleep a night and my sleep scores went up pretty quickly.

Most people begin mitochondrial‑support peptides between ages 35–50. ​[archived internal link]
Why that window?

Because that’s when mitochondrial efficiency naturally declines in a measurable way:

NAD+ levels drop ~1% per year after age 25

AMPK activity declines

VO₂ max decreases

Recovery slows

Muscle fatigue sets in faster

Insulin sensitivity decreases

Sleep quality becomes more fragile

By the mid‑30s to 40s, these changes become noticeable in:

Workout endurance

Ability to recover

Energy stability

Fatigue resistance

Muscle quality

Metabolic flexibility

This is the age range where peptides like MOTS‑c , NAD+ precursors , AOD‑9604 , GH secretagogues , and mitochondrial cofactors start to make a functional difference.

 

[domin8brix]

I was taking 5mg of Creatine a day, and I am a few weeks shy of 48. My bloodwork is actually spectacular, as long as I don't touch creatine, apparently. But NAD did nothing for me, unfortunately. I dug into all the others you've mentioned but haven't pulled the trigger on implementing.

 

[Skidude]

Great info I found 15mg motc a week was great on its own so this must be dynamite from reading the potential synergistic effects, could you tell me how you got on with the protocol any sides/ benefits?

Thank you

I agree with Calm Logic here:

Lots of people feel the same way with most of the non-GLP peptides.

The biggest problem with the non-GLPs is they distract from hoarding GLPs, haha.

Although there may be benefits, beyond anecdotal, primarily my motivation was to replenish energy from an extremely restrictive diet and substantial change in my normal dietary routine.

It is my understanding that as you age and hold many vices it is more likely that you might benefit; similar to someone taking B12 that has a deficiency. YMMV.

I'm an old guy that has drank and smoked for many years...

I will likely run another cycle in 3-6 months, but don't expect the drastic changes that you get from GLP-1s

 

[Nerker]

Question: I reconned 1000mg NAD+ with 4ml bac water.

I filtered and ended up with 5ml of liquid.

Does NAD+ has a volume increase after recon?

The fluid itself definitely seems thicker than my BPC, Mots or Reta fluids.

 

[Ajejebrazorf]

Question: I reconned 1000mg NAD+ with 4ml bac water.

I filtered and ended up with 5ml of liquid.

Does NAD+ has a volume increase after recon?

The fluid itself definitely seems thicker than my BPC, Mots or Reta fluids.

You basically added 4mg to a 1mg, seems normal end up with 5 mg/ml solution

 

[Nerker]

You basically added 4mg to a 1mg, seems normal end up with 5 mg/ml solution

Thanks. I reconned again with 6ml and and had about 7.5 ml of solution.

 

[dancs]

I did the NAD+ with the full MITO mix: ss-31/MOTSc/NAD+

I did 100 mg MWF

for the term: see below

View attachment 19222

I did take TMG to replenish Methyl

Practical Recommendations for NAD+ & TMG Supplementation ​

Timing and Dosage

Based on current knowledge, TMG’s role as a long-acting methyl donor means it can be taken at any time of the day . There’s no evidence to suggest that TMG must be taken at the same time as NAD+. A typical dosage is 1,000mg of TMG daily , which should be more than enough to support methylation needs. However, for individuals who find daily TMG supplementation burdensome, taking it only on the days they use their NAD+ patch is also a viable option.

Daily vs. Occasional Supplementation

While some patients prefer to take TMG daily, others opt to only take it when using their NAD+ patches. Both methods are acceptable, and individual responses can vary based on personal methylation capacity.

Methylation Testing

Unfortunately, there aren’t yet precise tests to measure methylation efficiency or NAD+ metabolism in a way that would inform personalized TMG dosing. However, genetic testing for MTHFR mutations and measuring homocysteine levels can provide insight into your methylation capacity.

The Takeaway ​
While the precise timing and the amount of TMG patients should use with NAD+ are not set in stone, our approach is based on what seems to work for most people: either take TMG daily or on the days when you use NAD+ supplements, especially if you have known methylation issues. You can easily find TMG supplements at online retailers or most vitamin and supplement shops.

At Sellers website, we’ll continue to monitor the latest research and provide updated guidance as more is understood about the interplay between NAD+ and methyl donors like TMG.

Taking Trimethylglycine (TMG) alongside NAD+ precursors (like NMN or NR) helps replenish methyl groups, which are depleted during the metabolic process of raising NAD+ levels. This combination supports optimal methylation , crucial for DNA repair, detoxification, and preventing high homocysteine, often taken together in a 1:1 ratio.

Key Aspects of Combining TMG with NAD+ Boosters:

Why Combine Them: NAD+ boosters help energy production but can drain the body’s methyl group supply. TMG is a "methyl donor," restoring these groups to keep methylation pathways open.

Optimal Ratio & Dosage: Experts, including Dr. David Sinclair, often recommend a 1:1 ratio of TMG to NMN/NR, commonly around

View attachment 19219 When to Take: TMG can be taken in the morning, often with food to maximize absorption and prevent stomach discomfort.

View attachment 19221

of TMG daily.

Synergy: While NMN/NR directly raises NAD+, TMG ensures the metabolic process does not cause long-term depletion of methyl groups, supporting, overall, cellular health.

Benefits: Potential benefits include sustained energy, improved cardiovascular health, and better methylation, with significant improvements often reported after 3-6 months.

@Calm Logic led me here. He gave you credit and here I am, about to buy some TMG.

Thank you!