过滤和污染。
- Thread starter articfox
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感谢您提出一些我也有的疑问。articfox said:
我还没进行过任何过滤。当我尝试研究过滤到底是什么时，我看到的第一件事就是把肽放进另一个容器里，不管是什么容器，但这样一来它就不是无菌的了。就像楼主说的那样。幸运的是，密封的小瓶……是密封的。我从不储存或重复使用针头。所有东西都会用酒精擦拭。没有东西会重复使用。一想到要把液体从密封的小瓶里取出来，我就浑身起鸡皮疙瘩。我相信肯定有非常巧妙的方法（楼主似乎暗示过这些方法，但我对楼主提到的一些内容只有非常模糊的概念）。
我的问题是：过滤的意义何在？假设你已经完美地完成了过滤（虽然我个人非常怀疑自己是否能做到完美），那么过滤的好处是什么？我从中国买来的未经过滤的灰色肽中可能残留哪些毒素？
Would you be willing to share what "all the equipment" entails?articfox said:Ive got all the equipment, but Im an arctic cat
Your best to study this topic and research it heavily, I am not comfortable giving you information when honestly I am just a novice. Use the search function and look on youtubetimj382 said:
Fair enough. Here's why I'm asking. Unless you have a lab and a laminar flow hood (I have nothing like that nor do I have access to it), this process sounds fraught af. Also, even though you may strain out bacteria that somehow snuck into the vial, and even if you do have a laminar flow hood, the bigger fear of endotoxins is not solved by filtering. Here's what I found in the research I'm doing and feel free to set me straight because I'm relatively new to this too. "Only specialized positively charged filters (like 'Mustang E' or 'Charged Durapor') can trap endotoxins via electrical attraction."articfox said:Your best to study this topic and research it heavily, I am not comfortable giving you information when honestly I am just a novice. Use the search function and look on youtube
I have a kitchen counter and a fridge. And if I had this intricate equipment, I would almost certainly screw it up. I think, at least for me, the dangers introduced by filtering far outweigh the dangers filtering is trying to remove. Again, anyone knowledgeable, please feel free to correct me. I'm trying to learn and I genuinely welcome the correction.
But what I think I might be starting to find out is that filtering is pretty much just not worth it for the vast majority of us who aren't scientists to begin with.
timj382 said:
Main reasons peptides are filtered
1. Remove bacteria and contaminants
Peptides are often reconstituted with bacteriostatic or sterile water.
Filtering (usually with a 0.22-micron sterile filter) helps remove bacteria and particulates that may have been introduced during handling or mixing.
This is especially important if the peptide is being stored and reused.
2. Improve sterility after reconstitution
Even if the peptide powder was sterile, the moment it’s mixed, sterility can be compromised.
Filtering reduces infection risk by acting as a final sterilization step.
3. Remove undissolved particles
Some peptides don’t fully dissolve or may contain microscopic solids from manufacturing or vial stoppers.
Filtering prevents these particles from remaining in solution.
4. Reduce injection-site reactions
Particulates or bacterial contamination can cause redness, swelling, or irritation.
Filtered solutions are generally smoother and better tolerated.
5. Quality control for research accuracy
In lab settings, contaminants can interfere with experiments and data.
Filtering helps ensure consistency and repeatability.
Appreciate that comprehensive and very useful answer. Thanks for taking the time.articfox said:
I'm not a pharmacy, the second I decided to do grey, I accepted, this is not sterile, it never will be.articfox said:
I do filter though, I do use 10 or more needles per recon, and I practised sterile tecniques in my earlier work life because of operating on patients, so I do feel ok safe.
There have been several rounds of sterility testing done on the commonly available brands of vials (available retail) advertised as sterile. While you can never be sure that every vial in the package you bought is sterile, it does at least give you an idea on a manufacturer’s track record.timj382 said:
As others have stated, the topic of filtering is covered very frequently and searching that keyword will give everyone hours worth of reading to enjoy.
So, I use a clear plastic tub on its side. Many years ago I use to grow mushrooms and dealt with spores, syringes and cultures. If you fuck up and contaminated the batch, it takes over quickly and ruins all your work.
So without a flow hood, you want the air extremely still. I use the bin so as little as possible drifts through the air downward and lands on my syringe/vial. One of the tricks is to move quickly as possible but in s steady measured movement. The less time any thing that may contam a vial.or syringe the better.
Really, i think a serious contamination in your vial is pretty visible. Once shit starts growing in a vial once you hold it up to the light youll.see it. And if your unsure, let the vial.sit a bit more and see if whatever is inside grows larger.
I opt not to filter, its extra steps, extra chance of contam, and apparently I like to live dangerously. If I hold that vial up and see anything in it thats not clear, such as a black speck, or even white specks, i will and have tossed vials. They are relatively cheap, this is a hobby and i could care less, will just buy more.
So without a flow hood, you want the air extremely still. I use the bin so as little as possible drifts through the air downward and lands on my syringe/vial. One of the tricks is to move quickly as possible but in s steady measured movement. The less time any thing that may contam a vial.or syringe the better.
Really, i think a serious contamination in your vial is pretty visible. Once shit starts growing in a vial once you hold it up to the light youll.see it. And if your unsure, let the vial.sit a bit more and see if whatever is inside grows larger.
I opt not to filter, its extra steps, extra chance of contam, and apparently I like to live dangerously. If I hold that vial up and see anything in it thats not clear, such as a black speck, or even white specks, i will and have tossed vials. They are relatively cheap, this is a hobby and i could care less, will just buy more.
Appreciate this pov. I think this may be where I land for now too.Gr33dyOctopus said:
Same with pen cartridges, a group recently sent 5 different brands for sterility testing, they all passed.Dos-Dox said:
When you only use pens, all that filtering takes is a 0.22um filter and an extra needle.
PeptideTest has a great YT video on that.
Oh man, I was all for not filtering before...however, after reconning some famous orange cap T30 from a vendor here...starting feeling weird. Gas and bubble guts in the morning, nausea, some fatigue. I just chalked it up to higher doses of both Tirz and Reta. HOWEVER, this morning I noticed something different (apologies in advance...). Got some bad diarrhea, but that turning into something REALLY strange, clear poop with some blood. Looking it up, symptoms showed it wasn't clear, it was actually mucus. Looking up said symptoms, it brought me to bacillary dysentery.Gr33dyOctopus said:
I'll note that multiple T30 vials from different vendors failed sterility. I have so much orange cap T30 and so many vendors carry it. I'll be filtering from now on, as I feel like absolute shit.
Maybe less anal.sex or rimming??!?!?IshimaruKenta said:Oh man, I was all for not filtering before...however, after reconning some famous orange cap T30 from a vendor here...starting feeling weird. Gas and bubble guts in the morning, nausea, some fatigue. I just chalked it up to higher doses of both Tirz and Reta. HOWEVER, this morning I noticed something different (apologies in advance...). Got some bad diarrhea, but that turning into something REALLY strange, clear poop with some blood. Looking it up, symptoms showed it wasn't clear, it was actually mucus. Looking up said symptoms, it brought me to bacillary dysentery .
I'll note that multiple T30 vials from different vendors failed sterility. I have so much orange cap T30 and so many vendors carry it. I'll be filtering from now on, as I feel like absolute shit.
Yeah...you got me.Gr33dyOctopus said:
I honestly never put two and two together until I pooped clear mucus. Symptoms started happening shortly after I started using the orange cap T30. I still feel that not the entire batch from every vendor is bad, but filtering is now a must. I understand this is reta forum, but filtering will now be added.
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