Filtering Question...

[Pocket]

In order to properly depyrogenize the vials you seem to need a dry-heat oven (250°C (482°F)).

Potentially dumb question, but wouldn't a kitchen oven do that?

Mine will go up to 500F no problem. You would want to make sure its an electric oven and not a gas one though. Gas ovens do not do dry heat.

 

[eidos]

Tedvio, the Australian poster nailed the bac water and alcohol piece, that's the part most people get wrong. Bacteriostatic water inhibits new growth but doesn't kill what's already in the vial. Same problem with the alcohol swirl approach you described. Alcohol needs full coverage and adequate contact time to actually sterilize and you can't guarantee either by swirling it through a sealed vial.

The cleanest approach if you're filtering for sterility is to use vials that are sold pre-sterilized from the manufacturer with documented sterilization method. Steam autoclaved, gamma irradiated, or dry heat at 170C for 60 minutes. That info should be on the listing. If it's not listed, treat the vial as not sterile.

Pen cartridges from reputable suppliers come sealed sterile and the packaging usually has a sterility indicator. That's a solid option if you don't want to deal with stoppers and crimpers.

If you're committed to sterilizing your own, a pressure cooker can work but you need to control temperature and time properly. Dry heat in an oven at 170C for 60 minutes also works for glass vials but the stoppers can't go in the oven, those need either irradiation or steam autoclave.

One thing to keep in mind, the .22 micron filter is what actually sterilizes your solution. The vial just needs to be sterile when it receives the filtered liquid. So the sterility question is really about the vial and stopper, not the peptide itself.

170°C isn't enough to make it pyrogen-free; you need at least 250°C, if I remember correctly.

Sterilizing equipment is quite an art. In the lab, I got to watch the technician do it a couple of times, but I was never allowed to do it myself, even if it was just for my own equipment. It takes skill, practice, and the right equipment to do that.

 

[Imcody2010]

Can anoyone point me towards a link/forum discussing filtering? I can take a look around but always appreciate someone who had one they like in mind. Thanks!

 

[HereKittyKitty]

will message you a link to a good video. Not sure if Zippity likes us to link out.

 

[Gr33dyOctopus]

Tedvio, the Australian poster nailed the bac water and alcohol piece, that's the part most people get wrong. Bacteriostatic water inhibits new growth but doesn't kill what's already in the vial. Same problem with the alcohol swirl approach you described. Alcohol needs full coverage and adequate contact time to actually sterilize and you can't guarantee either by swirling it through a sealed vial.

The cleanest approach if you're filtering for sterility is to use vials that are sold pre-sterilized from the manufacturer with documented sterilization method. Steam autoclaved, gamma irradiated, or dry heat at 170C for 60 minutes. That info should be on the listing. If it's not listed, treat the vial as not sterile.

Pen cartridges from reputable suppliers come sealed sterile and the packaging usually has a sterility indicator. That's a solid option if you don't want to deal with stoppers and crimpers.

If you're committed to sterilizing your own, a pressure cooker can work but you need to control temperature and time properly. Dry heat in an oven at 170C for 60 minutes also works for glass vials but the stoppers can't go in the oven, those need either irradiation or steam autoclave.

One thing to keep in mind, the .22 micron filter is what actually sterilizes your solution. The vial just needs to be sterile when it receives the filtered liquid. So the sterility question is really about the vial and stopper, not the peptide itself.

Im buying these.... like 60 of them

Traced them back to manufacturer firegene. Didn't see a mention of how they are sterilized?

 

[BNLFL]

Im buying these.... like 60 of them

Traced them back to manufacturer firegene. Didn't see a mention of how they are sterilized?

Good, Nice of you to think of me. I'll message my address.

 

[on2jinm]

Im buying these.... like 60 of them

Traced them back to manufacturer firegene. Didn't see a mention of how they are sterilized?

You might also want to check if they're made for injection purposes, and specifically if they have self-healing stoppers. I couldn't find much info about them on their website and it looks like they're made for a very specialized purpose, and they don't mention if they're rated for multiple needle punctures, unless I missed it.

 

[Gr33dyOctopus]

You might also want to check if they're made for injection purposes, and specifically if they have self-healing stoppers. I couldn't find much info about them on their website and it looks like they're made for a very specialized purpose, and they don't mention if they're rated for multiple needle punctures, unless I missed it.

Yea im super curious about them too, I think with shipping it was around 84 bucks, so no big loss if I cant use them. Hoping someone here can chime in on them.

 

[Gr33dyOctopus]

Hmmm

 

[Gr33dyOctopus]

You might also want to check if they're made for injection purposes, and specifically if they have self-healing stoppers. I couldn't find much info about them on their website and it looks like they're made for a very specialized purpose, and they don't mention if they're rated for multiple needle punctures, unless I missed it.

I been talking to my ai about it, and she said these are probably mid tier and likely miles better than the k2's on amazon. I wish id bought the more expensive ones now, but think these will work for now miles better than the Amazon k2s.

 

[Sasquatch]

So you want to filter. You gotta have a lurelock syringe to attach a filter. Draw up the recon'd dose, swap filter for a 31g needle, and pin. The more you fuck with it, the more chances of introducing bad shit. Is everyone screwing around because you want to use these pens? I'm not a f'ing doctor, but count the steps.

I realize Gr33dy gets 750mg in a single vial. That's different.

 

[Gr33dyOctopus]

So you want to filter. You gotta have a lurelock syringe to attach a filter. Draw up the recon'd dose, swap filter for a 31g needle, and pin. The more you fuck with it, the more chances of introducing bad shit. Is everyone screwing around because you want to use these pens? I'm not a f'ing doctor, but count the steps.

I realize Gr33dy gets 750mg in a single vial. That's different.

It's the best way for a price break!!!

7500mg kits are the bees knees!!!!!