Endotoxin levels

[Bigdog0628]

I'm having a hard time understanding Endotoxin levels. What is a safe level or what is the threshold of what I should be reading on a test?

 

[cheesecake]

from a different thread:

[archived internal link]

Reference: ~5 EU/kg/hour (≈350 EU/hour for a 70 kg person, IV setting)

Key differences:

That limit assumes slow IV exposure

A subcutaneous injection is still a bolus relative to that standard , just absorbed more gradually.

So:

~100–150 EU → near or below conservative limits

~250–450 EU → approaching or exceeding what’s typically allowed in controlled settings (especially as a bolus-equivalent exposure)

Step 3: What symptoms are likely at these levels?​[archived internal link]
~100–150 EU per injection​[archived internal link]

Many people: no obvious symptoms

Some:
Mild fatigue

Slight headache

Subtle heart rate increase

~200–300 EU per injection​[archived internal link]

Noticeable in a subset of users:
Mild “off” feeling within a few hours

Occasional chills or temperature sensitivity

Heart rate bump (e.g., +10–20 bpm)

~400+ EU per injection​[archived internal link]

More likely to produce a clear pyrogenic response :
Chills / mild fever

Headache

Fatigue

Elevated heart rate

Possible lightheadedness (mild BP drop)

 

[Grogu]

From Sufa Leeway over at FPPG on TG.

Thoughts on Endotoxin test results

docs.google.com

 

[Bigdog0628]

From Sufa Leeway over at FPPG on TG.

Thoughts on Endotoxin test results

docs.google.com

Thank you this helps a lot

 

[Grogu]

Thank you this helps a lot

Glad you found it helpful. It’s very conservative advice, but solid in my opinion. It provides a framework to consider your own risk tolerance.

 

[Bigdog0628]

Glad you found it helpful. It’s very conservative advice, but solid in my opinion. It provides a framework to consider your own risk tolerance.

Yes I was just under the impression purity and quantity were the biggest factors so thanks

 

[Calm Logic]

Last summer, I thought it would be cool to get T10, so I could recon the 10 mg of tirz with sterile water for immediate injection of basically the whole thing. With my endo and sterility concerns now, that would be a little crazy.

And there can be intra-batch variability with endos, as with sterility and everything else.

NAD+ is the leader of the pack for endos, at least lately. Most NAD+ still tests fine but some are crazy high. (So another reason to avoid large doses of NAD+, to avoid IM use of NAD+, and/or to get endo results for NAD+. Using oral precursors can also lower the amount of NAD+ needed for injection, as can the supplement apigenin.)

 

[Bigdog0628]

Last summer, I thought it would be cool to get T10, so I could recon the 10 mg of tirz with sterile water for immediate injection of basically the whole thing. With my endo and sterility concerns now, that would be a little crazy.

And there can be intra-batch variability with endos, as with sterility and everything else.

NAD+ is the leader of the pack for endos, at least lately. Most NAD+ still tests fine but some are crazy high. (So another reason to avoid large doses of NAD+, to avoid IM use of NAD+, and/or to get endo results for NAD+. Using oral precursors can also lower the amount of NAD+ needed for injection, as can the supplement apigenin.)

So if you are ordering t10 and t20 and t30 would you have to test all them or shouldn’t they all come from same batches?

 

[BNLFL]

So if you are ordering t10 and t20 and t30 would you have to test all them or shouldn’t they all come from same batches?

That'll all be different batches from what I've seen.