BAC water vs sterile water

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krsct

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Below is a link to an interview with Peter Janoshik of the testing company by the same name. Interesting to hear how he got into this business and some of his insights. Of interest, he states without hesitation that bacteriostatic water is not necessary for mixing peptides. Says sterile water can be used. Admits that it is a controversial opinion.

View: https://youtu.be/shgk3-u51Ys?si=4jl4kcH7ynyHYl6L
 
Not controversial ?

You aren't using bacteriostatic water for "mixing" but rather to inhibit microbiological growth in your vial.

People talk about using sterile water for peptides all the time. This is even used a lot by people where getting bac water is difficult.

Your other consideration for reconstitution is pH as certain peptides are a little more sensitive than others. In which case you may need AA and I've seen some people talk about using a few drops of DMSO for some substances though that's way outside my experience.
 
krsct said:
Below is a link to an interview with Peter Janoshik of the testing company by the same name. Interesting to hear how he got into this business and some of his insights. Of interest, he states without hesitation that bacteriostatic water is not necessary for mixing peptides. Says sterile water can be used. Admits that it is a controversial opinion.

View: https://youtu.be/shgk3-u51Ys?si=4jl4kcH7ynyHYl6L

Sterile water could be okay for reconstitution with the right peptide if all of the newly reconstituted peptide is used right away. Sterile water is easily contaminated, especially after inserting instruments or exposing it to air.

Bac water on the other hand has benzyl alcohol that inhibits bacterial growth, so reconstituted peptides with bac water are less likely to grow bacteria when stored.

Benzyl can deplete so its not the holy grail for extended time frames, and not all bac waters are equal but thats a story for another time like the bacteria growing in your fridge 😀
 
yrrdead said:
Not controversial ?

You aren't using bacteriostatic water for "mixing" but rather to inhibit microbiological growth in your vial.

People talk about using sterile water for peptides all the time. This is even used a lot by people where getting bac water is difficult.

Your other consideration for reconstitution is pH as certain peptides are a little more sensitive than others. In which case you may need AA and I've seen some people talk about using a few drops of DMSO for some substances though that's way outside my experience.
Its controversial because people keep recon peps for a month or more, and thats where bac is needed. Saline is used for single immediate use. Some experts suggested Peter needed to stay in his lane a little more...
 
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