ARA-299 cloudy after reconstitution + SLU-PP-332 solubility question

[cjdilan]

Looking for some input from people who’ve actually worked with these.

I reconstituted both with 2 mL bacteriostatic water.

ARA-299 (left vial): came out milky/cloudy. Let it sit overnight and it improved slightly but still not clear. No large particles, just overall turbidity.

SLU-PP-332 (right vial): initially had some haze/settling but now looks almost fully clear and stays clear when gently swirled. Maybe a tiny speck but nothing obvious.

Questions:

For ARA-299:

Is persistent cloudiness normal with BAC?

Has anyone had better results using PBS or another buffer instead?

At what point do you consider it a bad vial vs just slow dissolve?

For SLU-PP-332:

I know it’s not very water-soluble — does “clear in BAC” actually mean it’s properly dissolved, or just suspended?

Anyone running it injectable vs oral — what solvent system are you using?

General:

Do you guys wait 24–48 hrs before judging clarity?

Any tricks besides dilution that actually help (without getting into sketchy solvents)?

This way proofed by AI

 

[lessthanhalf]

In theory SLU is not soluble in water, it is a very polar molecule, so is only soluble in lipid like substances or dmso, There is no definite safe way to dissolve it for injection in humans that I know of, as dmso is not regarded as safe for human ingestion or injection. I would expect it to be cloudy in water, but maybe the suspension is too fine to see with the naked eye, the mannitol which is the usual filler would dissolve just fine, the SLU is in very tiny amounts which might explain why it is not cloudy. Chatgpt might be able to give some advice but it also might refuse to answer if it thinks you are asking for self dosing advice. Could it be injected s/c as a suspension? Maybe if it was well mixed? Not something that is normally done. It would presumably be absorbed in the subcutaneous tissues which I would assume have better solubility characteristics than water for polar substances, but cannot say for sure. Certainly not recommending it. My reading of the research says 5mg is likely a placebo dose anyway, going on mouse doses of 50mg/kg/dose. Which might be a good thing as it is not really well studied or understood enough to be ready for human testing yet. Once it has at least phase 1 testing in humans it would be interesting to look at again, as the effects in rodents sound interesting.

Have not looked up solubility of ara-290.

 

[Lovedog100]

I think that you know the answer for the cloudy vial. Not for human consumption. The SLU-pp-332 I think that you know the answer to that as well. Just about everything that I've read or seen says not to inject it. Oral seems the way to go. It's so much easier as well.

 

[AlexSilver]

Looking for some input from people who’ve actually worked with these.

I reconstituted both with 2 mL bacteriostatic water.

ARA-299 (left vial): came out milky/cloudy. Let it sit overnight and it improved slightly but still not clear. No large particles, just overall turbidity.

SLU-PP-332 (right vial): initially had some haze/settling but now looks almost fully clear and stays clear when gently swirled. Maybe a tiny speck but nothing obvious.

View attachment 20644

Questions:

For ARA-299:

Is persistent cloudiness normal with BAC?

Has anyone had better results using PBS or another buffer instead?

At what point do you consider it a bad vial vs just slow dissolve?

For SLU-PP-332:

I know it’s not very water-soluble — does “clear in BAC” actually mean it’s properly dissolved, or just suspended?

Anyone running it injectable vs oral — what solvent system are you using?

General:

Do you guys wait 24–48 hrs before judging clarity?

Any tricks besides dilution that actually help (without getting into sketchy solvents)?

This way proofed by AI

Your PH is off in the ARA-290. Add some acetic acid to balance the PH and it will clear right up.

 

[Jfrick11]

Looking for some input from people who’ve actually worked with these.

I reconstituted both with 2 mL bacteriostatic water.

ARA-299 (left vial): came out milky/cloudy. Let it sit overnight and it improved slightly but still not clear. No large particles, just overall turbidity.

SLU-PP-332 (right vial): initially had some haze/settling but now looks almost fully clear and stays clear when gently swirled. Maybe a tiny speck but nothing obvious.

View attachment 20644

Questions:

For ARA-299:

Is persistent cloudiness normal with BAC?

Has anyone had better results using PBS or another buffer instead?

At what point do you consider it a bad vial vs just slow dissolve?

For SLU-PP-332:

I know it’s not very water-soluble — does “clear in BAC” actually mean it’s properly dissolved, or just suspended?

Anyone running it injectable vs oral — what solvent system are you using?

General:

Do you guys wait 24–48 hrs before judging clarity?

Any tricks besides dilution that actually help (without getting into sketchy solvents)?

This way proofed by AI

I first add 0.5ml of PBS to ARA290 until it dissolves, then add 1.0ml BAC.

 

[HidingInPlainView]

I've been looking into this as well. I ordered Phosphate Buffered Saline as a precaution. My vendor reported no reconstitution complaints so far so maybe it will be fine with just Hospira? Hard to say, I haven't reconstituted it yet. I may order A.A. as a precaution as well.