3毫升预清洗注射笔小瓶：有什么想法？

[Romulusguy]

在开始尝试肽类药物的最初几周，我使用的是Easy Touch胰岛素注射针。仅仅几次注射后，我就克服了对注射的恐惧和焦虑。然而，我很快发现注射笔的剂量更精准，而且完全无痛，现在我把所有重组肽都转移到3毫升的注射笔小瓶中。这个过程相当简单，只需按照 peptidecritic网站上的优秀指南操作 即可，但如果操作正确且安全，可能会造成一些浪费。你需要一根针来吸取重组肽，一根用于新笔芯小瓶的排气针，以及一根用于将液体注入笔芯小瓶的针。小瓶装满并装入注射笔后，我插入针头，然后“排出”空隙，直到几滴液体从针头末端喷出。

今天，我偶然发现了这些经过蒸汽灭菌的“预排空”小瓶： https://www.pen-depot.com/products/...d-3-ml-long-stopper-pen-cartridges-single-use

虽然这听起来很合理，而且似乎是一个不错的选择，但我很好奇大家对于这种长条形开口的污染可能性有何看法。随着内部活塞在压力作用下向下移动，这个开口很快就会充满液体。Peptidecritic 在他的 DIY 预排空视频 中也谈到了这个过程及其潜在的污染风险，但是 https://youtu.be/6xH0pgkeLVo Peptidecritic 的视频中讨论的是 DIY 方法，而我讨论的这些小瓶是在预排空后进行灭菌的。

所以我的问题是：对于这些经过灭菌和预排空的小瓶，其污染风险是高于、低于还是等于我目前使用多根（干净/新鲜的）针头进行排气并转移到“未排空”小瓶的方法？也就是说，与我目前灌装未经净化处理的3毫升笔形药瓶的方法相比，这些预先净化处理的无菌药瓶是否会增加污染无菌内部环境的风险？您怎么看？

 

[randompersonrandom]

我试过先排气再注油的方法，这里有些人似乎挺喜欢这种方法的。不用给烟弹排气确实方便一些，也省了不少事，但我后来放弃了这种方法，又改回了排气的方式。我已经做了过滤，也尽力保持无菌，如果因为往已经暴露在空气中的烟弹里注油而可能前功尽弃，那就太傻了，所以我个人选择还是别冒险尝试了。

 

[javajoe316]

Thanks @Romulusguy and @randompersonrandom I just purchased some v4 pens that are coming today, and was sort of going through the process in my head. It does seem wasteful, to use 1 luer lock for recon, 1 filter, 1 luer lock, 1 syringe for venting to transfer to new vial. Then like you said a pen needle to purge air.

If you were loading 2 new pen vials, you can't really reuse any of these, right?

 

[Romulusguy]

I tried pre-purge-then-fill, which some people here generally like. It was nice to not have to vent the cart and a little less hassle, but I quit it and went back to venting the cart and not doing that. I'm already filtering and working really hard to keep sterile, it seems silly to maybe un-do that by filling into a cart that's been exposed to the air, so my personal choice is to not mess around too hard.

Those are my sentiments as well. I always filter after reconstituting, and I am BIG on safety/cleanliness. My only hesitation with completely dismissing these cartridges is the sterilization: I'm wondering if it's any riskier using these than, say, the "risk" of using multiple needles that have (albeit briefly) been exposed to the air, for venting and transfer, when using non-purged vials.

 

[randompersonrandom]

Thanks @Romulusguy and @randompersonrandom I just purchased some v4 pens that are coming today, and was sort of going through the process in my head. It does seem wasteful, to use 1 luer lock for recon, 1 filter, 1 luer lock, 1 syringe for venting to transfer to new vial. Then like you said a pen needle to purge air.

If you were loading 2 new pen vials, you can't really reuse any of these, right?

I do reuse if it's the same peptide in the same session. It's going from in a sterile cart that was wiped down with alcohol to a sterile cart that was wiped down with alcohol. When I do KLOW, it's 12 ml, so 4 carts; one vent needle gets used for all four, and I use a 13mm filter that will do the entire amount.

 

[Dilbert4Life]

I'm about to order some needles and syringes for preparing cartridges. What size needles and syringes do you get?

 

[Romulusguy]

I'm about to order some needles and syringes for preparing cartridges. What size needles and syringes do you get?

For standard insulin needles: I use the 1ml Easy Comfort 31G 1cc 5/16". I get mine from the diabetic warehouse. These are the BEST insulin needles you can buy.

For the 3ml pen cartirdges, you can really use any size needle for venting; I have been using a 20 gauge for venting. To draw out the solution and transfer, I use an 18 gauge. Don't forget your 13mm filters!

 

[Estarossa19]

I'm about to order some needles and syringes for preparing cartridges. What size needles and syringes do you get?

I went with a kit from underground supply and was happy with the results. I'm going to go ahead and just buy a bunch in bulk from amazon now that I have an understanding of whats needed. Comes with some hospira as well.

 

[desinr-gal]

对于标准胰岛素注射针：我用的是 1毫升的Easy Comfort 31G 1cc 5/16英寸 针头。我是在糖尿病用品商店买的。这些是你能买到的最好的胰岛素注射针。

对于3毫升的胰岛素笔芯，排气时其实可以用任何尺寸的针头；我一直用的是 20号针头 。抽取和转移药液时，我用的是 18号针头 。别忘了准备 13毫米的过滤器 ！

注意：一项研究表明，针头规格低于 22 号（我猜是）会导致针芯脱落，也就是针头橡胶塞的芯材脱落后进入肽溶液中。

我个人建议不要低于 25 号。另外，进针时要倾斜一定角度，这样也能降低脱落的风险。

 

[yrrdead]

注意：一项研究表明，针头规格低于 22 号（我猜是）会导致针芯脱落，也就是针头橡胶塞的芯材脱落后进入肽溶液中。

我个人建议不要低于 25 号。另外，进针时要倾斜一定角度，这样也能降低脱落的风险。

小瓶破碎和芯部剥落——可避免的威胁 - PMC

pmc.ncbi.nlm.nih.gov