Unable to inject meds.

[indolent]

the fact that freezing them fucks them up.

... kindly link to evidence of this

if Jano was the whiz that you think he is

Who then do you rely on for purity and mass testing before injecting these same chemicals into your system?

 

[raw_oyster_eater]

i would be OK freezing reconstituted material. you just don't want to break glass. i guess i could do an experiment and see if the product is still effective after freezing.

i have some Pt141, oxytocin and tesa i could give a try.

 

[GimmeABreak]

i would be OK freezing reconstituted material. you just don't want to break glass. i guess i could do an experiment and see if the product is still effective after freezing.

i have some Pt141, oxytocin and tesa i could give a try.

I think the peptide matters. Tirz had almost no loss after four freeze/thaw cycles. Epithalon was also tested and lost 9% after one cycle and 18% after four cycles.

I'm no scientist but these results might be related to the length of the peptide chain with shorter chains being less hearty than longer chains (like Tirz).

 

[raw_oyster_eater]

I'm no scientist but these results might be related to the length of the peptide chain with shorter chains being less hearty than longer chains (like Tirz).

intuitively, i'd think the longer molecules would be more vulnerable.

triz is a big one 4800g/mole

 

[AndyPanda]

... kindly link to evidence of this

FAQs | Ozempic® (semaglutide) injection & pill

Find answers to frequently asked questions about Ozempic® (semaglutide) injection and pill, including prescribing and patient resources. Read Important Safety and Prescribing Information, including Boxed Warning.

www.novomedlink.com

Wait, Do You Actually Need to Refrigerate Ozempic?

Ozempic is an injectable weight loss medication that must be stored properly to ensure safety. New pens must be kept in the fridge until ready for use.

www.health.com

 

[indolent]

FAQs | Ozempic® (semaglutide) injection & pill

Find answers to frequently asked questions about Ozempic® (semaglutide) injection and pill, including prescribing and patient resources. Read Important Safety and Prescribing Information, including Boxed Warning.

www.novomedlink.com

Thank you. Those are instructions, not scientific evidence of any sort.

And they're for the mechanical injector device (which everyone agrees shouldn't be frozen), but also subsequently incorporated in the Lilly Direct single-dose vials.

 

[raw_oyster_eater]

the pharmacists are pretty committed to keeping it in the fridge.

 

[AndyPanda]

Considering what he makes on each test and the sheer volume of business, I somehow I think Jano is probably doing ok working out his little underground lab.

The referenced Tirz test is N=1 data, but is pretty encouraging for Tirz (but not necessarily other peps) to be fine after a couple of freeze/thaw cycles.

After seeing the data I took a couple of vials of compound, that I will probably never use, and stuck them in my -20C freezer. They are from October and while freezing them may cause some degradation, it can't be worse than the vials sitting in the fridge for >6-12 months.

I’m sure Jano is doing fine financially, but let’s call a spade a spade. He is doing tests for illicit substances and he is not an accomplished scientist who has been peer reviewed or published. If you look into him there are plenty of people discrediting him on MesoRX, Reddit, etc.

Freezing the peptides causes crystallization which can alter them on a molecular level, making them dangerous. Keeping liquid in the fridge is totally different. That’s just a risk of degradation and bacteria growth.

 

[indolent]

Freezing the peptides causes crystallization which can alter them on a molecular level, making them dangerous.

"Lyophilizer machines, also known as freeze dryers, can reach temperatures as low as -40°C to -80°C (-40°F to -112°F) for freezing the product, and condensers can reach -50°C to -105°C (-60°F to -180°F). "

 

[hexagonal]

"Lyophilizer machines, also known as freeze dryers, can reach temperatures as low as -40°C to -80°C (-40°F to -112°F) for freezing the product, and condensers can reach -50°C to -105°C (-60°F to -180°F). "

While I believe the janoshik testing on freezing tirz, this post indicates you should be a bit more careful before you act as an authoritative source.

You'll notice there is a key word in your quote - dryers. Part of the lyophilization process involves creating a vacuum and drying out the vial as part of how it works. This is a key part of the process, otherwise they wouldn't need to use freeze dryers instead of just any other ultra-cold freezing method.

Why is it a key part of the process? Because it is known that "wet" freezing can damage the structure of many molecules - explicitly because of the crystallization AndyPanda is mentioning. While tirz in specific looks to be OK, we know that freezing/thawing HGH, for example, when it has moisture in the vial can and does cause damage, resulting in insoluble aggregates forming. Epitathlon was mentioned up thread. There are many many others as well.

 

[AndyPanda]

"Lyophilizer machines, also known as freeze dryers, can reach temperatures as low as -40°C to -80°C (-40°F to -112°F) for freezing the product, and condensers can reach -50°C to -105°C (-60°F to -180°F). "

Lyophilization is done under vacuum and it removes ice crystals through sublimation. Manufactures also use cryoprotectants to mitigate damage that are removed through the process. Additionally, during lyophilization the liquid freezes at controlled pace.

 

[GimmeABreak]

I’m sure Jano is doing fine financially, but let’s call a spade a spade. He is doing tests for illicit substances and he is not an accomplished scientist who has been peer reviewed or published. If you look into him there are plenty of people discrediting him on MesoRX, Reddit, etc.

Freezing the peptides causes crystallization which can alter them on a molecular level, making them dangerous. Keeping liquid in the fridge is totally different. That’s just a risk of degradation and bacteria growth.

Jano conducted the freeze/thaw test in accordance with the directions provided to him by the big group testing server. As I previously stated, this is an N=1 test but I would like to see more tests to confirm these results and would happily contribute to them, but this is the only test that I am aware of for Tirz.

I'm not sure why Lilly says not to freeze Mounjaro/Zepbound pens but I could see the freeze expansion process causing the pen to leak or the stopper to be forced out of the cartridge in these pens.

 

[fetefille]

I’m trying to help a friend by introducing him to compound semaglutide. I found him a website with a good price and directed him to Amazon for accessories. He’s about 6 injections in and complaining that he’s unable to depress the plunger to dispense the medicine. I’ve had issues like this when Luer lock needles weren’t screwed on tight enough, but this is a one piece syringe. Any ideas what might be going on?

If he got the syringes from Amazon, that may be the problem. I normally order from a diff site, but was in a hurry. Ordered 2 boxes of my normal brand. First box felt and operated as normal. This second box has a distinctly different feel in my hand and the plunger requires so much more force. I mean, I have to use both hands once it’s filled and inserted to depress it.

So maybe he’s got a counterfeit box like mine. I knew it was a risk, it’s not the end of the world, and I was warned. I just won’t do it again.

 

[keangkong]

When I have too much positive or negative pressure I find injecting a syringe without the needle will balance out. This venting effect allows me to try again by injecting equal air to my next withdraw. So i vent , then an example would be to inject .5 ml for a 50 unit dose. then extract my 50 units.

please let others comment on this, but it seems to work for me.

Do you mean injecting the syringe without the plunger?

 

[raw_oyster_eater]

I'm not sure why Lilly says not to freeze Mounjaro/Zepbound pens but I could see the freeze expansion process causing the pen to leak or the stopper to be forced out of the cartridge in these pens.

isn;t that glass in there?

 

[Skidude]

Do you mean injecting the syringe without the plunger?

That is correct, I also misunderstood the issue and the entire response was flawed.

Funny enough I noticed the second part the next morning, I just now recognize the "without the needle portion" so yes, vent without plunger or use a luer lock needle. my mistake

 

[GimmeABreak]

isn;t that glass in there?

It is, its very similar to the carts we use in our pens but has a needle affixed to it.

 

[Mykrodot 1111]

I’m trying to help a friend by introducing him to compound semaglutide. I found him a website with a good price and directed him to Amazon for accessories. He’s about 6 injections in and complaining that he’s unable to depress the plunger to dispense the medicine. I’ve had issues like this when Luer lock needles weren’t screwed on tight enough, but this is a one piece syringe. Any ideas what might be going on?

As you can tell folks definitely have an opinion on this one. You are going to have to dig deep and decide what you are comfortable with. I would start with a Google search for “storage of reconstituted peptides freeze”, there is a lot of info out there. Best wishes.

 

[noggin]

When I have too much positive or negative pressure I find injecting a syringe without the needle will balance out. This venting effect allows me to try again by injecting equal air to my next withdraw. So i vent , then an example would be to inject .5 ml for a 50 unit dose. then extract my 50 units.

please let others comment on this, but it seems to work for me.

How on earth do you inject a syringe without the needle

 

[keangkong]

How on earth do you inject a syringe without the needle

OP later clarified that to refer to injecting without the plunger. I had correctly guessed that plunger was what was intended, but only did so because I also inject the syringe without the plunger to eliminate any discrepancies in air pressure.