Thoughts on 90 day stability test results

[Notafed]

I reference this video

View: https://youtu.be/lI4am0U49N4

First I really wish someone would do a study retesting lyophilized Tirzepatide and Retatrutide after long term freezer storage. I would love to see if someone has a study referenceing a different peptide, but as this video shows different peptides and there structures can have different levels of stability.

Second, I question this study vs Eli Lilly and compounding. Because my understanding is Eli Lilly puts a shelf life on the drug of 2 years. Many compounders did the research to extend there bud's towards a year. Is it possible even if Tirzepatide is Tirzepatide structurally that different vendors could put out product with different levels of stability? Plenty of people on the compounding side aren't reporting issues you would expect to be hearing about if there effectiveness was being cut in half.

Is it an issue of only using Bacterial Static Water vs Phenol and/or other stabilizers. Did they leave these vials under bright light? Really want to hear from those more educated in this than I. Will this change anyones opinion especially those on low doses using high dose vials and pinning way past 28 days?

 

[308fan]

The Tirz held up really well. I am glad to see some interest in finding out. I was wondering about the Mots and now I know.

 

[Gt3294a]

I reference this video

View: https://youtu.be/lI4am0U49N4

First I really wish someone would do a study retesting lyophilized Tirzepatide and Retatrutide after long term freezer storage. I would love to see if someone has a study referenceing a different peptide, but as this video shows different peptides and there structures can have different levels of stability.

Second, I question this study vs Eli Lilly and compounding. Because my understanding is Eli Lilly puts a shelf life on the drug of 2 years. Many compounders did the research to extend there bud's towards a year. Is it possible even if Tirzepatide is Tirzepatide structurally that different vendors could put out product with different levels of stability? Plenty of people on the compounding side aren't reporting issues you would expect to be hearing about if there effectiveness was being cut in half.

Is it an issue of only using Bacterial Static Water vs Phenol and/or other stabilizers. Did they leave these vials under bright light? Really want to hear from those more educated in this than I. Will this change anyones opinion especially those on low doses using high dose vials and pinning way past 28

It seems consistent to me with the Jano tests that peptides hold up well. But Jano tests were about room temp powder if I recall correctly. I would like to see some tests on freezer peptides and fridge HGH. I could only test a year back though. Maybe in 2 years I will know.

 

[Chili777]

We already know that freezing Reta is not a good idea.

Can I have some advice on kit strength?

Go to PeptideDosages.com and review the information about Tirzepatide. It will give you a lot of information, including peptide purchasing information. The link below is for T5. https://peptidedosages.com/single-peptide-dosages/tirzepatide-5-mg-vial-dosage-protocol/

glp1forum.com

 

[Gt3294a]

We already know that freezing Reta is not a good idea.

Can I have some advice on kit strength?

Go to PeptideDosages.com and review the information about Tirzepatide. It will give you a lot of information, including peptide purchasing information. The link below is for T5. https://peptidedosages.com/single-peptide-dosages/tirzepatide-5-mg-vial-dosage-protocol/

glp1forum.com

That is after you reconstitute, no?

 

[Chili777]

That is after you reconstitute, no?

Correct. The freezer is the right place until then. It should keep for decades in the freezer.

 

[Gt3294a]

Correct. The freezer is the right place until then. It should keep for decades in the freezer.

I hope so. I got my own freezer in garage (I split a cow with 2 buddies every once in a while so need the space). Yet I will grab a vial and see that my wife squeezed a damn bag of frozen Costco nuggets on my peptide box. So I hope those babies are strong in the freezer.

 

[Chili777]

I hope so. I got my own freezer in garage (I split a cow with 2 buddies every once in a while so need the space). Yet I will grab a vial and see that my wife squeezed a damn bag of frozen Costco nuggets on my peptide box. So I hope those babies are strong in the freezer.

Can you put them on the bottom, out of the way? Peter Magic said any lyophilized peptide should last months at room temps, years in the fridge and decades in the freezer. I keep any I'm using in the next 6-12 months in my mini fridge, set at 4 out of 5 for cold. Protected in a hard case they should be fine, even with nuggets on top.

 

[BNLFL]

Can you put them on the bottom, out of the way? Peter Magic said any lyophilized peptide should last months at room temps, years in the fridge and decades in the freezer. I keep any I'm using in the next 6-12 months in my mini fridge, set at 4 out of 5 for cold. Protected in a hard case they should be fine, even with nuggets on top.

They better last decades based on my hoarding pile.

 

[alphaamigo]

The only thing I can think is, when did the stability go off the cliff? What was it at like 90 days. I with there were more people doing things like this.

 

[5byfive]

There is always a lot of talk about how long it is safe to use peptides after reconstitution but this isn't something I had really thought about. It seems for the GLPs, around 8 weeks is probably pretty safe from a degradation standpoint but it gets dicey after that. For myself, I always felt 8 weeks was a pretty good compromise safety wise but it seems its also a pretty good mark for stability.

 

[Airborne Daddy]

So use whatever you reconstitute in a month or so.... thought thats what we did anyway... never had a vial see 6 weeks. This study is pretty good news.

 

[Cannonball72]

https://peptidecritic.com/blog/peptide-degradation-and-storage-tirzepatide-mots-c-and-mazdutide-lab-results

Here’s a summary of the article:

Peptide Degradation & Storage: Lab Results from Peptide Critic x AFI

Peptide Critic partnered with Analytical Formulations Inc. to actually test how three peptides — Tirzepatide, MOTS-c, and Mazdutide — degrade after reconstitution, rather than relying on forum speculation.

How it was tested: Each peptide was stored in duplicate — one sample refrigerated at 36°F, one at ambient room temperature (~76°F) — reconstituted with bacteriostatic water (0.9% benzyl alcohol), kept in the dark, and measured over ~4 months using UV-Vis spectrophotometry.

Key findings by peptide:

• Tirzepatide held up surprisingly well short-term at room temp (97.8% activity at Day 7), but crashed to 40.3% by Day 31. Refrigerated, it held 92.3% at Day 61 but dropped to 40.1% by Day 113.

• MOTS-c debunked the “degrades in hours” myth — it retained 86.1% activity at ambient on Day 7. However, it fell sharply to 16.9% by Day 31 at room temp. Refrigerated, it held 88.9% at Day 31 but fell to 35.4% by Day 113.

• Mazdutide was the most room-temperature resilient early on, retaining 64.5% activity at Day 31 ambient. Refrigerated, it held 88.5% at Day 61 before dropping to 38.3% by Day 113.

The big pattern: All three peptides showed a similar curve — relatively slow initial decline, then a sharp drop-off later. This happened even in refrigerated samples, suggesting temperature isn’t the only factor. The benzyl alcohol in bacteriostatic water may be contributing to this threshold effect.

Bottom line:

• Refrigeration meaningfully extends usable life but doesn’t preserve peptides indefinitely

• Leaving something out briefly isn’t automatically ruinous, but it’s not a free pass either

• Different peptides degrade at different rates

• Once reconstituted, the clock is ticking faster than most people assume

 

[BNLFL]

So use whatever you reconstitute in a month or so.... thought thats what we did anyway... never had a vial see 6 weeks. This study is pretty good news.

My current R40 will be 8 weeks to almost gone,

 

[Habibibi]

We know from other degradation tests, and much better labs that tirzepatide doesn't degrade much after 90 days.

BTW, beware of AFI, they have failed blind tests. This is not surprising since they use the wrong machines to identify peptides anyway. Someone ought to put some kind of warning about that