Reliable method to reconstitute SLU-PP-332?

[Medex 35307]

Does anyone here have experience reconstituting SLU-PP-332 for subq? I ordered a kit without researching first, and now understand that it's hydrophobic, which means BAC water alone will just ruin it.

I watched a few YouTube vids that use .5ml DMSO then an equal amount of BAC water to reconstitute it, but everything I've read says this is a horrible idea, that injecting that concentration of DSMO is pretty toxic. I've read a couple sources that state using DMSO first, then PSB 80(tween 80), then phosphate buffered saline, in order to get the DSMO down to 1%, or close to 1%.

Anyone? Thanks for any help, and go ahead and laugh as necessary.

 

[Medex 35307]

Does anyone here have experience reconstituting SLU-PP-332 for subq? I ordered a kit without researching first, and now understand that it's hydrophobic, which means BAC water alone will just ruin it.

I watched a few YouTube vids that use .5ml DMSO then an equal amount of BAC water to reconstitute it, but everything I've read says this is a horrible idea, that injecting that concentration of DSMO is pretty toxic. I've read a couple sources that state using DMSO first, then PSB 80(tween 80), then phosphate buffered saline, in order to get the DSMO down to 1%, or close to 1%.

Anyone? Thanks for any help, and go ahead and laugh as necessary.

I know, I know, literally no one. But anyways, without linking to anywhere that wouldn't want association with self-described 'researchers,' I did finally stumble across this:

"Try to dissolve very hydrophobic peptides in a small amount of DMSO (30–50 μl, 100%), and then slowly add the solution dropwise to a stirring aqueous buffered solution such as PBS (or your desired buffer) to the required concentration. If the resulting peptide solution begins to show [cloudiness]turbidity, you have reached the limit of solubility. Sonication will help dissolve the peptides.

Rule of thumb:

0.1% DMSO is considered to be safe for almost all cells.

A final concentration of 0.5% DMSO is used widely for cell culture without cytotoxicity.

1% DMSO does not cause any toxicity in some cells, but 0.5% DMSO is recommended.

5% DMSO was used successfully in some cells."

Um, Still searching...

 

[Lovedog100]

Does anyone here have experience reconstituting SLU-PP-332 for subq? I ordered a kit without researching first, and now understand that it's hydrophobic, which means BAC water alone will just ruin it.

I watched a few YouTube vids that use .5ml DMSO then an equal amount of BAC water to reconstitute it, but everything I've read says this is a horrible idea, that injecting that concentration of DSMO is pretty toxic. I've read a couple sources that state using DMSO first, then PSB 80(tween 80), then phosphate buffered saline, in order to get the DSMO down to 1%, or close to 1%.

Anyone? Thanks for any help, and go ahead and laugh as necessary.

That's one of the reasons that I went with capsules. I don't know if they are better worse, however they are easier.

 

[390120]

ive read that some people have used 0.2mL or 0.3mL of DMSO to 2.3mL or 2.2mL of BAC with good results too

I guess you could use more BAC to reduce the DMSO concentration?

 

[Medex 35307]

I was considering whether this would waste a vial or not. Now I'll have to give it a try! Is there any way you could message me the links to where you read that? Thank you

 

[390120]

I was considering whether this would waste a vial or not. Now I'll have to give it a try! Is there any way you could message me the links to where you read that? Thank you

I’ve since lost where I read it, it was amongst my late night searches somewhere, im going to give it a try as well

 

[latviantower]

Pills be the way.