Is it feasible to split a reconstituted peptide into two vials?

[MG9550]

Hi everyone,

sorry if this has already been covered, I tried searching but couldn’t find anything, so apologies if I missed it.

Also, I should say upfront that I already have very strong doubts that my question might be a bit silly, but here we go anyway.

I have a vial of Tirz that needs to be reconstituted, and I would like to split the final solution into two separate vials (50/50).

Is this actually feasible without affecting sterility or integrity?

Any advice regarding sterility, handling, or other would be really appreciated.

Thanks a lot!

I do that for KLOW/GHK-Cu cartridges since heavier dilutions ease the sting of injection. I dissolve in 2 mL of Bac water, filter 1 mL of the reconstituted vial into 2 cartridges and then add Bac water to 3 mL into both cartridges.

Regarding sterility, I work in a Still Air Box that's meant for inoculating mushroom spores. Probably overkill, but as long as you sanitise all your vials and your work area and don't touch the rubber with your fingers after sanitising, you should be fine.

 

[Mara_aa]

I do that for KLOW/GHK-Cu cartridges since heavier dilutions ease the sting of injection. I dissolve in 2 mL of Bac water, filter 1 mL of the reconstituted vial into 2 cartridges and then add Bac water to 3 mL into both cartridges.

Regarding sterility, I work in a Still Air Box that's meant for inoculating mushroom spores. Probably overkill, but as long as you sanitise all your vials and your work area and don't touch the rubber with your fingers after sanitising, you should be fine.

Thanks so much for taking the time to explain your process.

In my case I wasn’t planning any further dilution, just splitting the reconstituted solution 50/50 into two sterile vials, so my main concern is exactly what you mentioned: maintaining sterility during the transfer.

I don’t have a setup like yours (no Still Air Box on my side), but it’s really helpful to know how you approach it, especially if I ever decide to go that route.

Really appreciate you sharing this!

 

[Mara_aa]

Do you mean using vent needle to remove all the air from the cartridge before freezing? Like priming the cartridge.

From what I understood, he means using a vent needle during filling to equalize pressure, so the plug doesn’t move.

When he says it’s “ok if the plug moves”, he’s referring to controlling it so it doesn’t travel too far or reach a non-sterile area.

Not talking about removing all the air before freezing or “priming”, it’s about pressure control during the fill.