呼吸道浑浊（我的血液酒精浓度很好）

[Chili777]

I don't frequent the other vendor TGs too much, but in my testing group I'm hearing that other providers are having the same kind of issues that suggest exactly what you are. I managed to dodge the bullet in advance by getting the R30, which hasn't had any reported problems. The TFA salts issue is the first real product related issue I think I've heard about with ERP other than the guy who bought the kits of Melanotan 2 and Mots-C, promptly got the the lids to the kits mixed up and subsequently shot 5mgs of MT2. Not ERP's fault of course, and fortunately he was ok.

I've made a few people in the community aware of it and Uzorak Labs is looking for samples vials to test. He (Alex) has the proper equipment to test for this. A regular Jano test won't catch it so purity, sterility, and weight all look fine. He just caught a contaminated batch of SS-31 from FSD.

It's no vendor's fault as this happened before they ever received the APIs so that's understood.

 

[ambot88]

I have ordered a vial or acetic acid to see if I can cure it with that. Just going to experiment at this point. I upgraded my replacement order to R30 to hopefully avoid more issue

 

[ambot88]

okay I found buffer solution on amazon - am I adding buffer solution to my BAC or reconning entirely with it?

 

[Camlbacker]

okay I found buffer solution on amazon - am I adding buffer solution to my BAC or reconning entirely with it?

According to the post above:

1. The Rescue: Draw 0.2mL to 0.5mL of sterile 1X PBS and add it to the cloudy vial. Gently swirl. The cloud should vanish in minutes.

2. Note: Recalculate your syringe units since your total volume is now higher!

You could also just add PBS bit by bit, until it clears. Just make sure you keep track of how much you're adding and adjust your dosing units accordingly.

 

[Chili777]

Article about exactly this issue:

https://open.suspicious-link-remove...?utm_source=share&utm_medium=android&r=7l77vf

 

[ambot88]

I bought some PBS from amazon and a it comes in a giant jug so any chance of sterility is gone, but it totally did clear up the reta. I will use it for my pin tomorrow and see if I live. yay science.

 

[Chili777]

I bought some PBS from amazon and a it comes in a giant jug so any chance of sterility is gone, but it totally did clear up the reta. I will use it for my pin tomorrow and see if I live. yay science.

I did some more research and it seems the peptide is fine after PBS. I wanted to know: where do the TFA salts actually go? I found a technical analysis of what the salts do to the peptide, and it sounds like nothing, once the environment is corrected. I'm not a chemist, so I ran it through an AI and got this summary:

1. Residual TFA is the hidden problem

• During peptide manufacturing, TFA (trifluoroacetic acid) is commonly used

• Some of it stays behind as TFA salts

• If not properly removed:

the peptide powder is acidic

⸻

2. When you add BAC water → pH crashes

• BAC water pH ≈ 5.7

• Add residual TFA → drops to ~4–4.5

That matters because:

• Reta’s isoelectric point (pI) ≈ 6

• Near that point → peptides lose charge

• When they lose charge → they clump together

Result:

cloudiness / flakes (“snowflakes”)

⸻

3. Salt and solvent effects worsen it

He explains three environments:

Filtered water

• No buffering → unstable → can crash

BAC water

• Alcohol reduces solubility

• pH too close to pI → aggregation

Saline

• “salting out” effect

• pushes molecules together → precipitation

⸻

His solution

Use PBS (phosphate-buffered saline)

Why?

• PBS buffers pH at ~7.4

• keeps you away from the pI danger zone

• neutralizes residual acid (TFA)

• stabilizes the peptide in solution

Your peptide is fine — it’s just crashing out because the environment is wrong.

Fix the environment → it stays clear.

 

[FarmgirlRebel]

I bought some PBS from amazon and a it comes in a giant jug so any chance of sterility is gone, but it totally did clear up the reta. I will use it for my pin tomorrow and see if I live. yay science.

Anyone have a solid source for vials of PBS smaller than those jugs?

I found one, but they sell only to labs and schools. I found one on a peptide site, but who knows if they just take it out of the big jug and put it into vials?

 

[Chili777]

You could always get the premixed powder and make a lifetime supply with sterile water. It's still a liter at a time though.

CryoKing 磷酸盐缓冲液 1X，PBS 预混粉，独立包装，pH 7.4，即溶型 PBS 粉末，适用于细胞或组织处理、仪器清洗，1 升/袋，5 袋装：亚马逊：工业与科学

CryoKing 磷酸盐缓冲液 1X，PBS 预混粉，独立包装，pH 7.4，即溶型 PBS 粉末，适用于细胞或组织处理、仪器清洗，1 升/袋，5 袋装：亚马逊：工业与科学

a.co

 

[ambot88]

我混合了另一瓶之前囤的试剂来比较澄清度，虽然PBS确实让它更清澈了，但并没有达到“正常”那瓶的澄清程度。我尝试直接加入大量PBS，看看需要多少才能澄清，结果发现澄清到一定程度后，再加PBS就没有任何效果了。我又尝试先加BAC再加PBS，以及先加PBS再加BAC，但结果都还是有点浑浊。我订购了一些醋酸，正在路上，我会用它来做实验。我已经接受了这套试剂盒对科学来说基本报废的事实。

不过，这种浑浊状态真的有危险吗？比如，如果只是残留的盐分，我还能把它固定住吗？我大概会这么做，之后会回来汇报结果。

 

[Chili777]

AA会加剧酸性，并杀死网状细胞。尝试少量多次地加入PBS缓冲液，直到溶液基本清澈，然后等待30分钟。这可能是最好的结果了。你试过这个方法吗？

 

[ambot88]

AA会加剧酸性，并杀死网状细胞。尝试少量多次地加入PBS缓冲液，直到溶液基本清澈，然后等待30分钟。这可能是最好的结果了。你试过这个方法吗？

我还没试过直接注射PBS，这样安全吗？它的保质期是多久？

 

[Chili777]

我还没试过直接注射PBS，这样安全吗？它的保质期是多久？

我上面的帖子说，PBS是唯一能稳定Reta环境并消除残留盐分的方法来纠正小瓶问题的方法。一旦完成这一步，Reta的安全性就达到了极限。我附上了关于如何解决盐分问题的完整评论。他建议用8 IU的苯甲醇(BA)代替BAC。除非你手头有BA，否则这不太可行，而且我们已经知道BAC没有帮助。所以这是一个权衡：保质期缩短，但肽的稳定性更高。我不知道你的剂量，但我用的是10毫克，所以肽的用量可以维持两周。你需要自己决定。

补充：还有一个重要的问题没有得到解答，那就是降低pH值会对Reta的降解产生什么影响。我们已经知道，在压力下，它比Tirz和其他肽更容易失去氨基键。只有通过测试才能回答这个问题。

 

[TooBigtoFail]

Based on posts from past 2 years, 'cloudy' due to pH, can be corrected. Some peptides require some AA to avoid the issue. Did you measure the pH after adding bac? and pH of the bac itself?

Hospira pH average is 5.7 but can vary between 4.5 to 7.0pH (this is from Pfizer's own tech sheet). They do not list using hydrochloric acid and/or sodium hydroxide for pH adjustment, thus the pH variation.

If your reta pH is also 'off', acidic, I am not surprise you get precipitation. Maybe first add Sodium Bicarbonate (not AA) to your bac to increase pH before you recon. Novo and E. Lilly list their excipients and I try to mimic their formula. Also, their reta has no Sodium Chloride. Tirz does list Sodium Chloride. Others have posted not to use BAC Sodium Chloride with reta as they reported cloudiness.

I have to say that my main reason for throwing away 6-8% of my vials in past 2 years was due to vials with no vacuum (CN suppliers). And in one of those case, the recon had become cloudy.

No more vacuum issues since I switched to Nex. Good luck. I would not use a cloudy recon (as mentionned on many medical sites). Not even after filtering it.

 

[ambot88]

I have a question about the AA save now, I added a drop on AA to my cloudy reta and immediately it kind of separated and had a bunch of visible particles but after about 15 minutes they had all dissipated and the solution was clear. Do you think the clumping ruined the reta or do you think it's okay now? The PBS rescued vials are not staying clear.

 

[Victorhouston]

I just got a kit of reta20 from a vendor I've used frequently - reconned the first vial and it was cloudy, shit happens was willing to let it slide, did a second vial, also cloudy. I am using a brand new vial of hospira bac. I then reconned an older vial just to make sure it wasn't my bac and it was crystal clear. I have contacted the vendor with pics and hope they make it right but even if they do, I'm now out of Reta and assume I'll weigh 300lbs by next month.

I had a similar scenario last month, after bumping around a few other sites and groups I found out others had encountered this same issue and a majority of those others actually still used it, I used it as well and it's still as effective as if it were to be clear but that's just me..

 

[Chili777]

I have a question about the AA save now, I added a drop on AA to my cloudy reta and immediately it kind of separated and had a bunch of visible particles but after about 15 minutes they had all dissipated and the solution was clear. Do you think the clumping ruined the reta or do you think it's okay now? The PBS rescued vials are not staying clear.

You can't know for sure without testing, but what I've read is that the Reta is still fine. I asked Gemini if AA would compromise the Reta and got the below. This is at up to one drop per mL, up to a 5% AA solution.

Will the Retatrutide be Compromised?

Generally, no. Retatrutide and similar GLP-1 analogues are relatively stable in slightly acidic environments.

Structural Integrity: Peptides are often stored as TFA salts specifically because they are more stable in an acidic state. Bringing the pH down to a range of 4.0 to 6.0 is standard for many injectable peptide formulations.

Bioavailability: Once injected, the body’s natural buffering system (the bicarbonate buffer) immediately neutralizes the small amount of acid, and the peptide performs as intended.

Injection Comfort: The primary "compromise" is not to the peptide, but to the user. A lower pH can cause a temporary stinging or burning sensation at the injection site.

 

[ambot88]

You can't know for sure without testing, but what I've read is that the Reta is still fine. I asked Gemini if AA would compromise the Reta and got the below. This is at up to one drop per mL, up to a 5% AA solution.

Will the Retatrutide be Compromised?

Generally, no. Retatrutide and similar GLP-1 analogues are relatively stable in slightly acidic environments.

Structural Integrity: Peptides are often stored as TFA salts specifically because they are more stable in an acidic state. Bringing the pH down to a range of 4.0 to 6.0 is standard for many injectable peptide formulations.

Bioavailability: Once injected, the body’s natural buffering system (the bicarbonate buffer) immediately neutralizes the small amount of acid, and the peptide performs as intended.

Injection Comfort: The primary "compromise" is not to the peptide, but to the user. A lower pH can cause a temporary stinging or burning sensation at the injection site.

Thanks chili! I'll give it a try and hope for the best

 

[ambot88]

I had a similar scenario last month, after bumping around a few other sites and groups I found out others had encountered this same issue and a majority of those others actually still used it, I used it as well and it's still as effective as if it were to be clear but that's just me..

so you just straight up used the cloudy reta and it was fine? good to know.

 

[Victorhouston]

so you just straight up used the cloudy reta and it was fine? good to know.

correct, will be taking my 3rd dose this Tuesday coming up and the weight is still steadily dropping