Empty sterile vials vials vials

[BNLFL]

We have many years worth of drugs! Shouldn't we have many years worth of supplies? I'd rather split the flat $25 shipping amongst many rather than buy smaller quantities and eat the $25 repeatedly.

Take a peak below. I'm covered. Doing math for my wife and I at 8mg is 16mg a week. Staying at this dose I have 14.5 years of just Reta in 302 or 322 vials. I'll have 32 vials of Hospira when my case shows.

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[Bioexplorer]

If you're really paranoid about vial sterility, why not just throw the cheapest vials you can find in an inexpensive pressure cooker for 20 minutes? Poor man's autoclave.

 

[AlexSilver]

If you're really paranoid about vial sterility, why not just throw the cheapest vials you can find in an inexpensive pressure cooker for 20 minutes? Poor man's autoclave.

Doesn't work for sealed vials.

 

[AlexSilver]

Sterile are common, pyrogen free is the next step up that you want.

They say you can get rid of pyrogens by just dry-cooking already sealed vials in a 250 degree oven for 30 minutes.

 

[Gr33dyOctopus]

They say you can get rid of pyrogens by just dry-cooking already sealed vials in a 250 degree oven for 30 minutes.

I just looked up pyrogen, hmmm. I definitely want pyrogen free vials...

 

[Bioexplorer]

Doesn't work for sealed vials.

Why not? I think almost all the vials we use are borosilicate glass and the butyl rubber stopper can survive the temperature no problem. The cap is probably also safe at 250F. I doubt the 15psi overpressure would be a problem. But I have a high risk tolerance and never tried this. Keep the vials above water of course.

Haven't used the pressure cooker for sterilization since my home brewing and yeast propagation days.

Pyrogens are a different issue. I don't think this process destroys endotoxins. But then again, I don't understand how endotoxins could be introduced during the vial manufacturing process.

 

[RetCurious]

They say you can get rid of pyrogens by just dry-cooking already sealed vials in a 250 degree oven for 30 minutes.

Link to "They" study please.

Because "They" usually don't know what they are talking about.

 

[AlexSilver]

Why not? I think almost all the vials we use are borosilicate glass and the butyl rubber stopper can survive the temperature no problem. The cap is probably also safe at 250F. I doubt the 15psi overpressure would be a problem. But I have a high risk tolerance and never tried this. Keep the vials above water of course.

Haven't used the pressure cooker for sterilization since my home brewing and yeast propagation days.

Pyrogens are a different issue. I don't think this process destroys endotoxins. But then again, I don't understand how endotoxins could be introduced during the vial manufacturing process.

It has nothing to do with the glass and rubber withstanding the temp.... it's because the vial is sealed.

I used to grow mushrooms and keeping things sterile is critical. In fact, it's hard for me to understand how they survive in the wild. Anyway, I learned by trial and error long ago. If the steam can't get to it, it doesn't get sterile. That's why sterilization pouches are made with a tyvek type material on one side.

Steam pressure sterilization does not destroy pyrogens

 

[AlexSilver]

Link to "They" study please.

Because "They" usually don't know what they are talking about.

I have no proof, That's why I didn't state as fact.... just what I have heard over the years in a local mushroom community.

 

[RetCurious]

I have no proof, That's why I didn't state as fact.... just what I have heard over the years in a local mushroom community.

Looks like you were right!

Heating methods are often used to ensure that glass and other lab equipment are free of pyrogenic material. Heat is applied by baking in a dry heat oven that is designed specifically for the depyrogenation process. Although endotoxins are relatively thermally stable, sufficient heating (250 °C for 30 min) results in a 3-log reduction of endotoxin levels.

Depyrogenation - Wikipedia

en.wikipedia.org

I think the next question is will the rubber bits survive this?

 

[AlexSilver]

I think the next question is will the rubber bits survive this?

Absolutely. The plastic caps will survive too.

 

[Bioexplorer]

It has nothing to do with the glass and rubber withstanding the temp.... it's because the vial is sealed.

I used to grow mushrooms and keeping things sterile is critical. In fact, it's hard for me to understand how they survive in the wild. Anyway, I learned by trial and error long ago. If the steam can't get to it, it doesn't get sterile. That's why sterilization pouches are made with a tyvek type material on one side.

Steam pressure sterilization does not destroy pyrogens

I am familiar with sterilization techniques. Steam contact is faster but dry heat at the same temperature can be just as effective. Just give it more time.

 

[AlexSilver]

I am familiar with sterilization techniques. Steam contact is faster but dry heat at the same temperature can be just as effective. Just give it more time.

If the temperature is high enough....

Been there, done that. I'm sorry, but given my experience with mushrooms, there is no chance I would ever believe something was sterile enough for injection into my body after only a low dry heat like kitchen oven temp.

 

[BNLFL]

I just looked up pyrogen, hmmm. I definitely want pyrogen free vials...

Spill the beans buddy.

 

[Gr33dyOctopus]

I ordered 120 of them an hour ago and shipping was $25. Sales tax was not included in the price for some reason though.

Hey! You want that deal for referring someone? Dming you

Spill the beans buddy.

I ended up picking up 60 of those vials, will review them once they get here i forgot the referal offer!

 

[ambot88]

I've just been using the amazon ones, I go through stuff pretty fast so I'm just not that concerned with sterility.

 

[medavaca]

I've bought both of the products mentioned by @birdwhacker and @Dos-Dox above and I can also vouch for the quality of the vials. Both vendors charge less than $2.00 per vial with discounts for larger quantities from at least one of them.

Don't be put off by the size of the vial they state; 2ml which is technically the "nominal fill amount". They actually hold a little more than 3ml and are the same physical size of every filled lyophilized peptide vial I've received from China.

Good to know! I have been frustrated looking for 3ml vials. I finally just settled on 3ml pen cartridges instead.