Do You Filter Your Peptides Before Use?

[trojanpeptide]

After reading this, I agree, it's wiser to filter sterilize. I just ordered a bunch of 4mm 0.22 micron PES filters and some sterile 5mL vials. I will recon in BAC and then filter to a new vial. Might even be a good way to split 30mg vials so that the second vial will have less traffic when you get to using it. Thanks for the suggestion.

 

[zpped]

Never have filtered, and never will. If there's evidence stating that these vials all dangerous and must be filtered, I'll do it. But there isn't any evidence stating otherwise. Extra careful, sure, but ain't nobody's got time for that.

What evidence do you need? Plenty of vials are contaminated in one way or another. Wether that's dangerous is a personal decision.

Why would he filter before testing? That completely defeats the purpose of purpose of the testing. I have never in my hours of research on janoshik of in my correspondence with the labs heard that stated or implied.

You must have a flawed understanding of what testing accomplishes. He absolutely filters before every mass/purity test to protect his machine.

 

[IshimaruKenta]

Costs… $1? Takes 5 min max? Cheap insurance. Maybe it just illusion of control bias. Idk.

I get that, it's just for me, I'm not seeing the benefit right now. Also, I have a 10 month old baby that I take care of, and she doesn't always let me have 5 minutes needed to recon something and then put it in a pen. I try to do it as quickly as possible because she needs attention (imagine that! lol). So adding 5 minutes to the process is a bit much for me right now. I don't discount the idea of filtering as a whole, nor do I feel anyone is wrong in doing so.

If I'm seeing enough evidence where I need to, I'll find some time. I'm 100% not like the other guy.

 

[deleted.user.18]

What evidence do you need? Plenty of vials are contaminated in one way or another. Wether that's dangerous is a personal decision.

You must have a flawed understanding of what testing accomplishes. He absolutely filters before every mass/purity test to protect his machine.

If you filter before a sterility test, what’s the point? I do not have a flawed under of testing, maybe for GCMS, LCMS, HPLC etc. but this discussion is about sterility- or maybe I have a flawed understanding of this discussion.

 

[pavlovs]

The evidence that there is stuff in our vials that shouldn't be in our vials is that Jano filters his peptides before testing so that the stuff in vials that isn't supposed to be in vials doesn't hurt his machines.

 

[keangkong]

Janoshik wrote: "Sterility generally is not an issue with lyophilizates. There are not too many microbes that can survive either lyophilization or months while lyophilized. Those that can are not quite likely to find their way into the vials." That's from https://chat.peppys.org/t/lab-faq-guide-with-janoshik-analytical/4136. Click on the .pdf document and you can read the whole thing if you belong to Peppys.

Edited: I didn't realize that the quotation used strikeout type. I fixed that.

 

[pavlovs]

Janoshik wrote: " terility generally is not an issue with lyophilizates. There are not too many microbes that can survive either lyophilization or months while lyophilized. Those that can are not quite likely to find their way into the vials." That's from https://chat.peppys.org/t/lab-faq-guide-with-janoshik-analytical/4136 . Click on the .pdf document and you can read the whole thing if you belong to Peppys.

That's pretty interesting, and that most of us put most of our peptides in the freezer might be helpful, too. However, I also don't want a tiny piece of cardboard, plastic or rubber stopper injected into my body. I think it just really comes down to risk tolerance and personal preference. I feel safer filtering than not filtering, but I don't think anyone is stupid for not filtering.

 

[desinr-gal]

Why would he filter before testing? That completely defeats the purpose of purpose of the testing. I have never in my hours of research on janoshik of in my correspondence with the labs heard that stated or implied.

IDK I heard it stated by members here, the reason was to prevent contamination or damage to his expensive equipment? I too thought it would completely change the results. Which is why I always filter.

 

[Jack brown]

Not sure of his actual stance on filtering though.

In his podcast, he advocated using sterile water, filtering, and keeping for 28 days.

 

[keangkong]

If you filter before a sterility test, what’s the point? I do not have a flawed under of testing, maybe for GCMS, LCMS, HPLC etc. but this discussion is about sterility- or maybe I have a flawed understanding of this discussion.

That does make sense. Filtering before a sterility test would render the test useless.

 

[keangkong]

In his podcast, he advocated using sterile water, filtering, and keeping for 28 days.

I did hear a podcast with Janoshik. He does prefer sterile water to BAC water. I don't recall him in the podcast I heard mention filtering. I do recall that when asked how long a person you use a peptide reconstituted with sterile water, he didn't say anything that specific. Granted we could have listened to different podcasts.

 

[IshimaruKenta]

He also uses "ultra pure" water for his sterility test, unless otherwise requested. Not sure what the difference is between the two, but they don't use sterile water for that test.

 

[keangkong]

The evidence that there is stuff in our vials that shouldn't be in our vials is that Jano filters his peptides before testing so that the stuff in vials that isn't supposed to be in vials doesn't hurt his machines.

If Janoshik filtered peptides before conducting a sterility test, then it seems like every sterility test would result in a negative.

 

[keangkong]

He also uses "ultra pure" water for his sterility test, unless otherwise requested. Not sure what the difference is between the two, but they don't use sterile water for that test.

He recommends "sterile water" for reconstituting peptides for people to use themselves.

 

[IshimaruKenta]

He recommends "sterile water" for reconstituting peptides for people to use themselves.

On his details page, he specifically states he uses "ultra pure" water when doing the sterility test, unless you request something else. I didn't just pull that out of nowhere

Peptides are by default dissolved in ultrapure water. 3 ml of ultrapure water are used by default for 3ml peptide vials.

If you want your sample to be dissolved in a different solvent, we need to be informed!

 

[zpped]

If you filter before a sterility test, what’s the point? I do not have a flawed under of testing, maybe for GCMS, LCMS, HPLC etc. but this discussion is about sterility- or maybe I have a flawed understanding of this discussion.

There is no filtering before a sterility test (there isn't really such a thing as a sterility test). Jano does a TAMC & TYMC test that does not involve filtering. And it's not uncommon for things to fail.

 

[zpped]

He recommends "sterile water" for reconstituting peptides for people to use themselves.

BAC water is extremely hard to source in Europe. Also, the MOST safe thing to do is use vials in single dose amounts with SWI (sterile water for injection)

If using the vial more than once it's absolutely better to use BAC (again, it's very hard to source it in Europe) so swi and use within 2 weeks is the next best things.

 

[IshimaruKenta]

Ultrapure water is a term commonly used in manufacturing to emphasize the fact that the water is treated to the highest levels of purity for all contaminant types, including organic and inorganic compounds, dissolved and particulate matter, and dissolved gases, as well as volatile and non-volatile compounds, reactive and inert compounds, and hydrophilic and hydrophobic compounds.

I'm guessing that's sterile?

 

[deleted.user.18]

I'm guessing that's sterile?

Sterile in his lab at that Laminar Airflow Workstation maybe. In our house the second you pierce that vial it’s most likely no longer sterile. We try our best not to introduce contaminants, bacteria etc. but the reality is unless you have a Laminar Airflow Workstation and clean room at home there’s a good chance it’s not sterile.

 

[IshimaruKenta]

Sterile in his lab at that Laminar Airflow Workstation maybe. In our house the second you pierce that vial it’s most likely no longer sterile. We try our best not to introduce contaminants, bacteria etc. but the reality is unless you have a Laminar Airflow Workstation and clean room at home there’s a good chance it’s not sterile.

Just wondering why he says he uses ultrapure water instead of sterile water for injection.