Centrifuge filtering?

[esdsafepoet]

Been reading more on filtering and the limitations of using 22micron syringe filters, namely lack of virus filtration. Anyone here used something like Amicon Ultra Centrifugal Filters? If so, what model centrifuge do you use and do you find fixed angle rotor vs swinging bucket makes a difference? And how about RCF? Is 2000xg enough, or is it better to go higher? Lastly, how safe is this type of filtering for the peptide? Thanks.

 

[Vicki]

Is this a major concern for synthetic peptides?

 

[esdsafepoet]

Probably not. Call it an academic exercise on my part.

 

[Morbius]

I see a lot of smaller centrifuges on Craigslist so if you want to try this out it may be possible at a relatively low cost.

(I was looking for bigger centrifuges for culinary uses -- no luck finding the right deal.)

 

[esdsafepoet]

View attachment 7136

I see a lot of smaller centrifuges on Craigslist so if you want to try this out it may be possible at a relatively low cost.

(I was looking for bigger centrifuges for culinary uses -- no luck finding the right deal.)

I've found a couple candidates if I really wanted to try this out. This one seems like a good balance of value and performance as it can get an RCF of 3000xg. The swinging bucket models are much more expensive, and refrigerated ones even more so, so I probably wouldn't go that route in any case.

In addition to the centrifuge and filters, I'd also need an appropriate pipettor. So the costs add up. It's interesting to consider if only from a science and process perspective. But there's also the nature of gray market products to consider.