It concerns me a bit that you are getting strong effects at doses lower than I would predict.
Using the extremely imperfect method of converting rodent doses to human of 1/10th the rodent dose in mg/kg, doses in rodent studies 10mg/kg-100mg/kg human eq is 1-10mg/kg which is 100mg to 1g/day for a 100kg human. I am not saying this is going to be accurate but it does usually produce ballpark results or at least the correct order of magnitude.
But from this calculation the low dose from the lowest dose studies in mice translates to 1mg/kg/day or 60 to 100mg/day in humans - all based on oral doses. I bothered to work all this out as the animal studies are interesting. But your responses indicate stronger effects at lower doses of about 1/30th to 1/12th the lowest dose I calculated. Given that the easiest test to do is heart rate, getting it to 100 sounds like an unsafe dose, but it is also possible for it to be placebo response, which is the whole problem with n of 1 studies with no blinding. It also suggests that dose finding phase 1 trials really need to be done before human use. Please do not use this to guide dosing, it sounds like the doses you are using are too high rather than too low going on heart rate. Online I have seen doses from 0.5mg to 200mg orally , this degree of dosing variation is very unusual. Sometimes the vendors sell the drugs in deliberately low doses if they think there might be reasons to worry about adverse effects. Most of the animal studies were done with oral dosing, and bioavailability is not super high as it is a very polar molecule but it is not so bad as to explain a 12-100x dose change from oral to parenteral.
I think if you are going to try it I would suggest being a bit more scientific in measuring effects, including especially body temperature, blood pressure and heart rate, both before doses to get a baseline and after. And weight . Any rise in body temperature is a serious problem, the dose is too high, as is an increase in heart rate above 10 bpm or increase in blood pressure. Hopefully after a lot of measurements , non drug effects like expectation or placebo effects would tend to go down and make the results a bit cleaner.
