At-Home Filtering

[deleted.user.18]

“There’s more than one way to skin a cat”

And

“Don't spoil the ship for a halfpenny of tar.”

Are two colloquialisms that come to mind from this whole discussion.

 

[desinr-gal]

-Remove any obvious bubbles by tapping; do not push fluid back and forth through the needle (reduces shear/adsorption risk)

I usually am reconning a 30mg peptide with 3ml bac in a 3ml vial. I cannot pre force 3ml of air into that, in order to draw out the solution on one go. I always have to do a seesaw, air in, peptide out, air in peptide out system until its all out. The only way to avoid that would be to only initially recon with 1-2ml bac and then, add more bac separately to the new vial/cart. Which, if I used that syringe to add back some of that bac into the luer lock as a flush it would work I suppose?

I reconned and filtered yesterday, I pre wetted the filter with bac, before starting recon. It worked fine. but when flushing more bac into the cart the filter seemed to blow out, whoosh and a ton of bubbles. I am not that strong, I dont push very hard. IDK why this happens to me.

 

[desinr-gal]

What if I did it this way? I am not mentioning the previously stated steps of wiping sterilizing etc. those are a given.. also I am trying to make use of one of the 100 hypodermic insulin syringes I have stockpiled,while trying to conserve the 15 luerlocks I have.

for 30mg in 3ml vial, going into new sterile vial or cart.

Use a hypodermic 30G syringe to draw bac water, 1-2ml to inject this into a 3ml luerlock syringe hub.

Add needle to luer lock, inject bac to recon vial only partially. let sit.

Use same hypo to draw rest of bac to equal 3.4 ml. use some to squirt into female side of filter, *inject half into new cart or vial.

Use luerlock to draw out recon sol. ** alternatively some bac water in hypo could be added to this syringe, (instead of into vial/cart) after drawing out recon for filtering.

attach filter to luerlock plus new needle. Filter recon pep into new vial/cart.

remove luerlock syringe, from filter with needle, add in rest of bac to luerlock syringe, re attach filter and finish injecting into cart ( could leave needle and filter inside cart instead)

what do you think?

 

[50ShadesGreyMarket]

-Remove any obvious bubbles by tapping; do not push fluid back and forth through the needle (reduces shear/adsorption risk)

I usually am reconning a 30mg peptide with 3ml bac in a 3ml vial. I cannot pre force 3ml of air into that, in order to draw out the solution on one go. I always have to do a seesaw, air in, peptide out, air in peptide out system until its all out. The only way to avoid that would be to only initially recon with 1-2ml bac and then, add more bac separately to the new vial/cart. Which, if I used that syringe to add back some of that bac into the luer lock as a flush it would work I suppose?

I reconned and filtered yesterday, I pre wetted the filter with bac, before starting recon. It worked fine. but when flushing more bac into the cart the filter seemed to blow out, whoosh and a ton of bubbles. I am not that strong, I dont push very hard. IDK why this happens to me.

If you’re filtering already why don’t you add a hydrophobic filter between a needle/syringe without the plunger and allow filtered air to vent into the vial as you draw out peptide? Even on the teeny tiny cartridge stoppers you have room for 2 needles. I use a 25g to filter the peptide and a 31g to create an air vent. This is how we did it in school, it’s worked for decades and it’s like 10 cents for another needle. If you’re filling multiple cartridges at once you can re-use the vent filter as long as you adhere to strict sterilization practices.

 

[50ShadesGreyMarket]

What if I did it this way? I am not mentioning the previously stated steps of wiping sterilizing etc. those are a given.. also I am trying to make use of one of the 100 hypodermic insulin syringes I have stockpiled,while trying to conserve the 15 luerlocks I have.

for 30mg in 3ml vial, going into new sterile vial or cart.

Use a hypodermic 30G syringe to draw bac water, 1-2ml to inject this into a 3ml luerlock syringe hub.

Add needle to luer lock, inject bac to recon vial only partially. let sit.

Use same hypo to draw rest of bac to equal 3.4 ml. use some to squirt into female side of filter, *inject half into new cart or vial.

Use luerlock to draw out recon sol. ** alternatively some bac water in hypo could be added to this syringe, (instead of into vial/cart) after drawing out recon for filtering.

attach filter to luerlock plus new needle. Filter recon pep into new vial/cart.

remove luerlock syringe, from filter with needle, add in rest of bac to luerlock syringe, re attach filter and finish injecting into cart ( could leave needle and filter inside cart instead)

what do you think?

Okay, I went ahead and loaded a cartridge and took some pics, these are 23g needles and as you can see there’s no issue as long as you keep the air filter in the headspace.

Ignore lack of cleanliness, this is for demonstration purposes only.

Pic 1: pulling bac

Pic 2: filling cartridge

Pic 3: expelling air

Pic 4: full cartridge

 

[50ShadesGreyMarket]

In Korea, Luer lock syringes are available, but Luer lock needles are not sold. I understand that ordering them from overseas is illegal. Can I use a regular syringe needle with a Luer lock syringe? This is for peptide filtration.

When you say the needles aren’t sold, is it a legality issue? I just ordered a bunch of 100 count boxes, 31g 8mm for about $10 ea w/free shipping from Shotlog. They now ship outside the US.

In order to get the needle locked to the Luer filter you need a Luer on each side. Check online and see if any shops ship to your country. They’re used in science labs all over the world, shouldn’t be too hard to source unless it requires a prescription. Good luck on your search!

 

[basedpepbae]

If you’re filtering already why don’t you add a hydrophobic filter between a needle/syringe without the plunger and allow filtered air to vent into to vial as you draw out peptide? Even on the teeny tiny cartridge stoppers you have room for 2 needles. I use a 25g to filter the peptide and a 31g to create an air vent. This is how we did it in school, it’s worked for decades and it’s like 10 cents for another needle. If you’re filling multiple cartridges at once you can re-use the vent filter as long as you adhere to strict sterilization practices.

Where do you recommend getting filters? Everywhere I’ve seen are closer to $1 or more a piece.

 

[50ShadesGreyMarket]

Where do you recommend getting filters? Everywhere I’ve seen are closer to $1 or more a piece.

I order mine from a lab supplier but I know they sell them at Locofiltr and Amazon. $1 each doesn’t sound too bad for Gamma sterilized PES .22 33mm. If you’re buying on the grey market you’re saving hundreds, if not thousands a year and filtering is cheaper than a trip to the ER. The whole point of my peptide journey is to improve my health and longevity, I don’t want to compromise that to save a few bucks here and there.

(I completely understand not everyone cares though.)

 

[basedpepbae]

I order mine from a lab supplier but I know they sell them at Locofiltr and Amazon. $1 each doesn’t sound too bad for Gamma sterilized PES .22 33mm. If you’re buying on the grey market you’re saving hundreds, if not thousands a year and filtering is cheaper than a trip to the ER. The whole point of my peptide journey is to improve my health and longevity, I don’t want to compromise that to save a few bucks here and there.

(I completely understand not everyone cares though.)

Yea no worries. I don’t mind paying my current price for filters. I’m only using Tirz so I don’t need as many anyway.

 

[50ShadesGreyMarket]

Yea no worries. I don’t mind paying my current price for filters. I’m only using Tirz so I don’t need as many anyway.

If you have any good recommendations or reviews I’d love to hear them!

 

[basedpepbae]

I purchased a basic pen kit and filters from Underground Supply. I would probably continue to purchase from them in the future. Carts and filters.

 

[desinr-gal]

Okay, I went ahead and loaded a cartridge and took some pics, these are 23g needles and as you can see there’s no issue as long as you keep the air filter in the headspace.

Ignore lack of cleanliness, this is for demonstration purposes only.

Pic 1: pulling bac

Pic 2: filling cartridge

Pic 3: expelling air

Pic 4: full cartridge

First Thank you for taking the time to do this and take pics! I see what you're saying, and I will look for some of those. I have been filtering with the PES 4mm . Acquiring supplies and shipping them to Hawaii, has gotten rather complicated and expensive. Can you suggest a source?

What about the idea of transferring bac water directly into the luerlock to flush etc?

 

[50ShadesGreyMarket]

First Thank you for taking the time to do this and take pics! I see what you're saying, and I will look for some of those. I have been filtering with the PES 4mm . Acquiring supplies and shipping them to Hawaii, has gotten rather complicated and expensive. Can you suggest a source?

What about the idea of transferring bac water directly into the luerlock to flush etc?

Oh gosh, I’ve heard Hawaii is expensive for everything. Do you have Amazon there? Look up 10 packs of Locofiltr, I think they’re about $10 USD for 10. Good luck and let me know if you want me to check other sources.

Yes I prime with bac so the edge absorption is mostly bac. I tested this once by pushing bac w/a drop of food coloring through the filter then pushing regular bac and the edges still had coloring.

 

[ContainHer]

learned endotoxin screening from an actual scientist who makes peptides in a lab for a living but I’m sure if I typed out the steps there would a lot of folks who disagree with the process.

There may be a lot of folks interested in learning how to do this....it's me, I'm a lot of folks. I would greatly appreciate a rundown on endotoxin testing at home.

 

[desinr-gal]

Oh gosh, I’ve heard Hawaii is expensive for everything. Do you have Amazon there? Look up 10 packs of Locofiltr, I think they’re about $10 USD for 10. Good luck and let me know if you want me to check other sources.

Yes I prime with bac so the edge absorption is mostly bac. I tested this once by pushing bac w/a drop of food coloring through the filter then pushing regular bac and the edges still had coloring.

I will look for those thanks.

 

[deleted.user.18]

There may be a lot of folks interested in learning how to do this....it's me, I'm a lot of folks. I would greatly appreciate a rundown on endotoxin testing at home.

I have been researching since @50ShadesGreyMarket posted about it. Seems like maybe some front end equipment buys but then the consumables may be $15-$20 per test after that.

 

[50ShadesGreyMarket]

I have been researching since @50ShadesGreyMarket posted about it. Seems like maybe some front end equipment buys but then the consumables may be $15-$20 per test after that.

For brevity I didn’t add all the other things like if you’re not pulling air back into the vial/cartridge then you can use a 31g without the plunger. It has less vacuum. I showed two 23’s and forgot to remove the plunger on the air filter because I was just staging pics and forgot that part.

 

[50ShadesGreyMarket]

There may be a lot of folks interested in learning how to do this....it's me, I'm a lot of folks. I would greatly appreciate a rundown on endotoxin testing at home.

There may be a lot of folks interested in learning how to do this....it's me, I'm a lot of folks. I would greatly appreciate a rundown on endotoxin testing at home.

Absolutely but I’m going to type into a text editor and double check that I didn’t leave anything out. My previous “how-tos” have been in the middle of something else and by memory so I failed to clarify a couple of steps and it was confusing for some. I want to make sure I get the endotoxin steps all included since it’s expensive to set up and important to get correct.

 

[Ruckus4519]

Okay, I went ahead and loaded a cartridge and took some pics, these are 23g needles and as you can see there’s no issue as long as you keep the air filter in the headspace.

Ignore lack of cleanliness, this is for demonstration purposes only.

Pic 1: pulling bac

Pic 2: filling cartridge

Pic 3: expelling air

Pic 4: full cartridge

First off, thank you for sharing your knowledge with us. I apologize for replying to an older thread. I’m currently going down the archives of this section, but this one was particularly dense with new information.

As I understand it, this is more than what a lot of folks do. I had already saved the first post for later, but seeing the pictures with the gloves and syringes made me realize just how out of my depth I would’ve been without them.

Some questions:

Is that a puppy pad?

Is the demonstration liquid dyed water?

You are using a pen? Is that because of daily administered ones? Do you have weekly ones?

Bonus question that I have yet to find an answer for:

Is there a maximum amount of fluid volume for subcutaneous injections? I assume if perhaps there is, that one simply moves to a different site.

 

[ForrestRun]

Absolutely but I’m going to type into a text editor and double check that I didn’t leave anything out. My previous “how-tos” have been in the middle of something else and by memory so I failed to clarify a couple of steps and it was confusing for some. I want to make sure I get the endotoxin steps all included since it’s expensive to set up and important to get correct.

Are you still willing to share some information about your at-home endotoxin testing? I tried to buy some kits that seemed fairly doable at home, but I wasn't able to because I'm just an individual and not associated with a business or research organization. I'd love to hear about your setup in general, I can do my own research from there if you don't want to write up a whole how-to!