Endotoxins!

[TheUnNatural]

Yeah, no.

The Difference Between Sterilizing Fluids and Endotoxin Removal

Get an understanding of the differences between sterilizing fluids and endotoxin removal. how is endotoxin created., removed, etc.

www.criticalprocess.com

 

[Habibibi]

The Difference Between Sterilizing Fluids and Endotoxin Removal

Get an understanding of the differences between sterilizing fluids and endotoxin removal. how is endotoxin created., removed, etc.

www.criticalprocess.com

Charged filters will also remove your peptide

 

[Riddi]

Good info

No.

The best way is high temperatures. Not applicable to these type of peptides

I think the only way for peptides would be by chromatography separation with anion exchange column... Expensive..

You probably will have more risk and problems with the lack of sterility than endotoxins..

For example

If the vial has for example 10 Eu of endotoxins total and you dilute in 10mL of BAC water and inject only 1mL of that you are injecting just 1EU every 5 or 7 days... Not a big problem and in the vial the quantity of endotoxins will be the same. They don't grow.. The concentration will always be the same

But when we are talking about bacteria, fungus, yeasts... And if you don't filter or use a low quality BAC water, they can grow.

On day one you can have 10 or 50 live cells and after a week the double or more

And also depend of the type... Some bacteria for example are not tottaly pathogenic and you don't have so much risks and there are bacteria highly pathogenic and can put you in the hospital..

That's why I mention

Filtering is reducing risk and for that you must buy good filters and good sterile vials

Hi Lyophilized, any suggestion on filtration level? is 0.22ug what is advisable? Also can you or anyone else suggest where one may source good sterile vials. I tried a few times via Amazon US and all were substandard/questionable quality and sent back. I've been seeking but striking out in that area. =( Thanks in advance!

 

[Lyophilized]

Hi.

Because I'm Biochemist I have access to filters that I don't know if are easy available to everyone...

The ones I use are these in the picture

Try to Google the part-number in the picture.

0.22um are enough, but I use 0.20um

They don't need to be 33mm diameter like I use... Can be much smaller, less "dead volume".

 

[Riddi]

Hi.

Because I'm Biochemist I have access to filters that I don't know if are easy available to everyone...

The ones I use are these in the picture

Try to Google the part-number in the picture.

0.22um are enough, but I use 0.20um

They don't need to be 33mm diameter like I use... Can be much smaller, less "dead volume".

Thanks for the information! i will look into it.